A Review on Biosynthesis of Silver Nanoparticles and Their Biocidal Properties

Overview

Journal J Nanobiotechnology

Publisher Biomed Central

Specialty Biotechnology

Date 2018 Feb 18

PMID 29452593

Citations 238

Authors

Khwaja Salahuddin Siddiqi

Azamal Husen

Rifaqat A K Rao

Affiliations

Soon will be listed here.

Abstract

Use of silver and silver salts is as old as human civilization but the fabrication of silver nanoparticles (Ag NPs) has only recently been recognized. They have been specifically used in agriculture and medicine as antibacterial, antifungal and antioxidants. It has been demonstrated that Ag NPs arrest the growth and multiplication of many bacteria such as Bacillus cereus, Staphylococcus aureus, Citrobacter koseri, Salmonella typhii, Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumonia, Vibrio parahaemolyticus and fungus Candida albicans by binding Ag/Ag with the biomolecules present in the microbial cells. It has been suggested that Ag NPs produce reactive oxygen species and free radicals which cause apoptosis leading to cell death preventing their replication. Since Ag NPs are smaller than the microorganisms, they diffuse into cell and rupture the cell wall which has been shown from SEM and TEM images of the suspension containing nanoparticles and pathogens. It has also been shown that smaller nanoparticles are more toxic than the bigger ones. Ag NPs are also used in packaging to prevent damage of food products by pathogens. The toxicity of Ag NPs is dependent on the size, concentration, pH of the medium and exposure time to pathogens.

Citing Articles

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References

Reddy A, Chen C, Chen C, Jean J, Chen H, Tseng M . Biological synthesis of gold and silver nanoparticles mediated by the bacteria Bacillus subtilis. J Nanosci Nanotechnol. 2010; 10(10):6567-74. DOI: 10.1166/jnn.2010.2519. View

Hosseini-Abari A, Emtiazi G, Ghasemi S . Development of an eco-friendly approach for biogenesis of silver nanoparticles using spores of Bacillus athrophaeus. World J Microbiol Biotechnol. 2013; 29(12):2359-64. DOI: 10.1007/s11274-013-1403-4. View

Ali K, Ahmed B, Dwivedi S, Saquib Q, Al-Khedhairy A, Musarrat J . Microwave Accelerated Green Synthesis of Stable Silver Nanoparticles with Eucalyptus globulus Leaf Extract and Their Antibacterial and Antibiofilm Activity on Clinical Isolates. PLoS One. 2015; 10(7):e0131178. PMC: 4489395. DOI: 10.1371/journal.pone.0131178. View

Shankar S, Rai A, Ahmad A, Sastry M . Rapid synthesis of Au, Ag, and bimetallic Au core-Ag shell nanoparticles using Neem (Azadirachta indica) leaf broth. J Colloid Interface Sci. 2004; 275(2):496-502. DOI: 10.1016/j.jcis.2004.03.003. View

Parikh R, Singh S, Prasad B, Patole M, Sastry M, Shouche Y . Extracellular synthesis of crystalline silver nanoparticles and molecular evidence of silver resistance from Morganella sp.: towards understanding biochemical synthesis mechanism. Chembiochem. 2008; 9(9):1415-22. DOI: 10.1002/cbic.200700592. View

Gulcin I, Topal F, Cakmakci R, Bilsel M, Goren A, Erdogan U . Pomological features, nutritional quality, polyphenol content analysis, and antioxidant properties of domesticated and 3 wild ecotype forms of raspberries (Rubus idaeus L.). J Food Sci. 2012; 76(4):C585-93. DOI: 10.1111/j.1750-3841.2011.02142.x. View

Mata R, Nakkala J, Sadras S . Catalytic and biological activities of green silver nanoparticles synthesized from Plumeria alba (frangipani) flower extract. Mater Sci Eng C Mater Biol Appl. 2015; 51:216-25. DOI: 10.1016/j.msec.2015.02.053. View

Bolla J, Alibert-Franco S, Handzlik J, Chevalier J, Mahamoud A, Boyer G . Strategies for bypassing the membrane barrier in multidrug resistant Gram-negative bacteria. FEBS Lett. 2011; 585(11):1682-90. DOI: 10.1016/j.febslet.2011.04.054. View

Jayachandra Reddy N, Nagoor Vali D, Rani M, Sudha Rani S . Evaluation of antioxidant, antibacterial and cytotoxic effects of green synthesized silver nanoparticles by Piper longum fruit. Mater Sci Eng C Mater Biol Appl. 2013; 34:115-22. DOI: 10.1016/j.msec.2013.08.039. View

10.

Chwalibog A, Sawosz E, Hotowy A, Szeliga J, Mitura S, Mitura K . Visualization of interaction between inorganic nanoparticles and bacteria or fungi. Int J Nanomedicine. 2011; 5:1085-94. PMC: 3023237. DOI: 10.2147/IJN.S13532. View

11.

Akhavan O, Ghaderi E . Toxicity of graphene and graphene oxide nanowalls against bacteria. ACS Nano. 2010; 4(10):5731-6. DOI: 10.1021/nn101390x. View

12.

Duran N, Marcato P, Alves O, de Souza G, Esposito E . Mechanistic aspects of biosynthesis of silver nanoparticles by several Fusarium oxysporum strains. J Nanobiotechnology. 2005; 3:8. PMC: 1180851. DOI: 10.1186/1477-3155-3-8. View

13.

Shankar S, Ahmad A, Sastry M . Geranium leaf assisted biosynthesis of silver nanoparticles. Biotechnol Prog. 2003; 19(6):1627-31. DOI: 10.1021/bp034070w. View

14.

Cronholm P, Karlsson H, Hedberg J, Lowe T, Winnberg L, Elihn K . Intracellular uptake and toxicity of Ag and CuO nanoparticles: a comparison between nanoparticles and their corresponding metal ions. Small. 2013; 9(7):970-82. DOI: 10.1002/smll.201201069. View

15.

Kokila T, Ramesh P, Geetha D . Biosynthesis of AgNPs using Carica Papaya peel extract and evaluation of its antioxidant and antimicrobial activities. Ecotoxicol Environ Saf. 2016; 134(Pt 2):467-473. DOI: 10.1016/j.ecoenv.2016.03.021. View

16.

Arunachalam K, Shanmuganathan B, Sreeja P, Parimelazhagan T . Phytosynthesis of silver nanoparticles using the leaves extract of Ficus talboti king and evaluation of antioxidant and antibacterial activities. Environ Sci Pollut Res Int. 2015; 22(22):18066-75. DOI: 10.1007/s11356-015-4992-7. View

17.

Dibrov P, Dzioba J, Gosink K, Hase C . Chemiosmotic mechanism of antimicrobial activity of Ag(+) in Vibrio cholerae. Antimicrob Agents Chemother. 2002; 46(8):2668-70. PMC: 127333. DOI: 10.1128/AAC.46.8.2668-2670.2002. View

18.

Kiran G, Sabu A, Selvin J . Synthesis of silver nanoparticles by glycolipid biosurfactant produced from marine Brevibacterium casei MSA19. J Biotechnol. 2010; 148(4):221-5. DOI: 10.1016/j.jbiotec.2010.06.012. View

19.

Oves M, Khan M, Zaidi A, Ahmed A, Ahmed F, Ahmad E . Antibacterial and cytotoxic efficacy of extracellular silver nanoparticles biofabricated from chromium reducing novel OS4 strain of Stenotrophomonas maltophilia. PLoS One. 2013; 8(3):e59140. PMC: 3605433. DOI: 10.1371/journal.pone.0059140. View

20.

Li N, Xia T, Nel A . The role of oxidative stress in ambient particulate matter-induced lung diseases and its implications in the toxicity of engineered nanoparticles. Free Radic Biol Med. 2008; 44(9):1689-99. PMC: 2387181. DOI: 10.1016/j.freeradbiomed.2008.01.028. View