Dental Pulp Stem Cell-derived Chondrogenic Cells Demonstrate Differential Cell Motility in Type I and Type II Collagen Hydrogels

Overview

Journal Spine J

Specialty Orthopedics

Date 2018 Feb 17

PMID 29452287

Citations 12

Authors

Li Yao

Nikol Flynn

Affiliations

Soon will be listed here.

Abstract

Background Context: Advances in the development of biomaterials and stem cell therapy provide a promising approach to regenerating degenerated discs. The normal nucleus pulposus (NP) cells exhibit similar phenotype to chondrocytes. Because dental pulp stem cells (DPSCs) can be differentiated into chondrogenic cells, the DPSCs and DPSCs-derived chondrogenic cells encapsulated in type I and type II collagen hydrogels can potentially be transplanted into degenerated NP to repair damaged tissue. The motility of transplanted cells is critical because the cells need to migrate away from the hydrogels containing the cells of high density and disperse through the NP tissue after implantation.

Purpose: The purpose of this study was to determine the motility of DPSC and DPSC-derived chondrogenic cells in type I and type II collagen hydrogels.

Study Design/setting: The time lapse imaging that recorded cell migration was analyzed to quantify the cell migration velocity and distance.

Methods: The cell viability of DPSCs in native or poly(ethylene glycol) ether tetrasuccinimidyl glutarate (4S-StarPEG)-crosslinked type I and type II collagen hydrogels was determined using LIVE/DEAD cell viability assay and AlamarBlue assay. DPSCs were differentiated into chondrogenic cells. The migration of DPSCs and DPSC-derived chondrogenic cells in these hydrogels was recorded using a time lapse imaging system. This study was funded by the Regional Institute on Aging and Wichita Medical Research and Education Foundation, and the authors declare no competing interest.

Result: DPSCs showed high cell viability in non-crosslinked and crosslinked collagen hydrogels. DPSCs migrated in collagen hydrogels, and the cell migration speed was not significantly different in either type I collagen or type II collagen hydrogels. The migration speed of DPSC-derived chondrogenic cells was higher in type I collagen hydrogel than in type II collagen hydrogel. Crosslinking of type I collagen with 4S-StarPEG significantly reduced the cell migration speed of DPSC-derived chondrogenic cells.

Conclusions: After implantation of collagen hydrogels encapsulating DPSCs or DPSC-derived chondrogenic cells, the cells can potentially migrate from the hydrogels and migrate into the NP tissue. This study also explored the differential cell motility of DPSCs and DPSC-derived chondrogenic cells in these collagen hydrogels.

Citing Articles

Effects of cell culture time and cytokines on migration of dental pulp stem cell-derived chondrogenic cells in collagen hydrogels.

Yao L, Flynn N, Kaphle P Physiol Rep. 2024; 12(18):e70063.

PMID: 39327065 PMC: 11427086. DOI: 10.14814/phy2.70063.

Selenium-SelK-GPX4 axis protects nucleus pulposus cells against mechanical overloading-induced ferroptosis and attenuates senescence of intervertebral disc.

Jia C, Xiang Z, Zhang P, Liu L, Zhu X, Yu R Cell Mol Life Sci. 2024; 81(1):49.

PMID: 38252317 PMC: 10803455. DOI: 10.1007/s00018-023-05067-1.

Effects of puerarin on the intervertebral disc degeneration and biological characteristics of nucleus pulposus cells.

Tang H, Zhang S, Lu X, Geng T Pharm Biol. 2022; 61(1):12-22.

PMID: 36524765 PMC: 9762855. DOI: 10.1080/13880209.2022.2147548.

Characterization of a Stemness-Optimized Purification Method for Human Dental-Pulp Stem Cells: An Approach to Standardization.

Dieterle M, Gross T, Steinberg T, Tomakidi P, Becker K, Vach K Cells. 2022; 11(20).

PMID: 36291072 PMC: 9600643. DOI: 10.3390/cells11203204.

Application of Biocompatible Scaffolds in Stem-Cell-Based Dental Tissue Engineering.

Tayanloo-Beik A, Nikkhah A, Roudsari P, Aghayan H, Rezaei-Tavirani M, Nasli-Esfahani E Adv Exp Med Biol. 2022; 1409:83-110.

PMID: 35999347 DOI: 10.1007/5584_2022_734.

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