Novel Automated Immune Turbidimetric Assay for Routine Urinary Cystatin-C Determinations

Overview

Journal Bioanalysis

Specialty Chemistry

Date 2018 Feb 17

PMID 29451000

Citations 5

Authors

Balazs Szirmay

Peter Kustan

Zoltan Horvath-Szalai

Andrea Ludany

Agnes Lakatos

Diana Muhl

Istvan Wittmann

Attila Miseta

Gabor L Kovacs

Tamas Koszegi

Affiliations

Soon will be listed here.

Abstract

Aim: There is no commercially available urinary cystatin-C (u-CYSC) test in the market. Therefore, we optimized and validated an automated immune turbidimetric test for u-CYSC measurements and investigated u-CYSC concentrations in acute and chronic diseases which might lead to renal tubular disorders.

Materials & Methods: A particle-enhanced immune turbidimetric assay was adapted and validated on a Cobas 8000/c502 analyzer. Urine samples of different patient groups were also analyzed.

Results: Our method showed excellent analytical performance. U-CYSC/u-creatinine (u-CREAT) was higher in sepsis-related acute kidney injury group (p < 0.001) compared with controls and to patients with chronic hypertension and Type 2 diabetes.

Conclusion: We validated a fast, sensitive, fully automated u-CYSC assay which is ideal for routine use and might be a potential complementary laboratory test to evaluate renal tubular function.

Citing Articles

Measurement of Urinary Gc-Globulin by a Fluorescence ELISA Technique: Method Validation and Clinical Evaluation in Septic Patients-A Pilot Study.

Koszegi T, Horvath-Szalai Z, Ragan D, Kosa B, Szirmay B, Kurdi C Molecules. 2023; 28(19).

PMID: 37836706 PMC: 10574505. DOI: 10.3390/molecules28196864.

Monitoring of Serological, Cellular and Genomic Biomarkers in Transplantation, Computational Prediction Models and Role of Cell-Free DNA in Transplant Outcome.

Jimenez-Coll V, Llorente S, Boix F, Alfaro R, Galian J, Martinez-Banaclocha H Int J Mol Sci. 2023; 24(4).

PMID: 36835314 PMC: 9963702. DOI: 10.3390/ijms24043908.

The Most Promising Biomarkers of Allogeneic Kidney Transplant Rejection.

Rogulska K, Wojciechowska-Koszko I, Dolegowska B, Kwiatkowska E, Roszkowska P, Kapczuk P J Immunol Res. 2022; 2022:6572338.

PMID: 35669103 PMC: 9167141. DOI: 10.1155/2022/6572338.

Novel Damage Biomarkers of Sepsis-Related Acute Kidney Injury.

Ragan D, Horvath-Szalai Z, Szirmay B, Muhl D EJIFCC. 2022; 33(1):11-22.

PMID: 35645693 PMC: 9092722.

Urinary actin, as a potential marker of sepsis-related acute kidney injury: A pilot study.

Ragan D, Kustan P, Horvath-Szalai Z, Szirmay B, Bugyi B, Ludany A PLoS One. 2021; 16(7):e0255266.

PMID: 34310652 PMC: 8312921. DOI: 10.1371/journal.pone.0255266.