, a Target of SlmiR482e-3p, Provides Resistance Against the Fungal Pathogen in Tomato

Overview

Journal Front Plant Sci

Date 2018 Feb 14

PMID 29434609

Citations 13

Authors

Hui-Min Ji

Min Zhao

Ying Gao

Xin-Xin Cao

Hui-Ying Mao

Yi Zhou

Wen-Yu Fan

Katherine A Borkovich

Shou-Qiang Ouyang

Peng Liu

Affiliations

Soon will be listed here.

Abstract

The vast majority of plant disease resistance () genes encode nucleotide binding site-leucine-rich repeat (NBS-LRR) proteins, which specifically determine the plant immune response and have been demonstrated to be targets of several microRNA (miRNA) families. The fungus f. sp. (FOL) causes vascular wilt disease in tomato worldwide. Here, we explored a possible role for in tomato defense against FOL. is a predicted NBS-LRR like gene that is targeted by slmiR482e-3p, a member of slmiR482 miRNA family. Northern blot data demonstrated that all seven members of the slmiR482 family were regulated in diverse ways after infection by FOL. The ability of to be regulated by slmiR482e-3p was confirmed at the transcript level by co-expression studies in . A virus-induced gene silencing (VIGS) approach revealed that confers resistance to the Motelle tomato cultivar. Taken together, our study has identified a novel gene, , which is targeted by slmiR482e-3p at the transcript level, and is necessary for resistance to tomato wilt disease .

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