The Influenza C Virus NS Gene: Evidence for a Spliced MRNA and a Second NS Gene Product (NS2 Protein)
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It has previously been shown that the shortest RNA (RNA 7) of influenza C viruses codes for a nonstructural (NSI) protein (Nakada et al. (1985) J. Virol. 56, 221-226). Experiments reported here indicate that RNA 7 also directs the synthesis of a second nonstructural protein via a spliced mRNA. The amino terminal 62 codons of this NS2 protein appear to be shared with the NS1 protein and the carboxyl terminal 59 amino acids are unique (derived from a +1 open reading frame in the mRNA). Although the size of the C virus NS2 protein is comparable to that of the A and B virus NS2 proteins, the overall arrangement of the C virus NS gene is quite different from that of the A and B virus NS genes. The second (+1) open reading frame of the C virus NS gene is completely overlapped by that of the NS1 protein, whereas the second (+1) open reading frame of the A and B virus NS genes extends to the 3' end of the RNA and only partially (or in some strains not at all) overlaps the NS1 open reading frame.
Sreenivasan C, Sheng Z, Wang D, Li F Pathogens. 2021; 10(12).
PMID: 34959538 PMC: 8704295. DOI: 10.3390/pathogens10121583.
Epidemiology and Clinical Characteristics of Influenza C Virus.
Sederdahl B, Williams J Viruses. 2020; 12(1).
PMID: 31941041 PMC: 7019359. DOI: 10.3390/v12010089.
Functional Characterization and Direct Comparison of Influenza A, B, C, and D NS1 Proteins and .
Nogales A, Aydillo T, Avila-Perez G, Escalera A, Chiem K, Cadagan R Front Microbiol. 2020; 10:2862.
PMID: 31921042 PMC: 6927920. DOI: 10.3389/fmicb.2019.02862.
Hemagglutinin-esterase-fusion (HEF) protein of influenza C virus.
Wang M, Veit M Protein Cell. 2015; 7(1):28-45.
PMID: 26215728 PMC: 4707155. DOI: 10.1007/s13238-015-0193-x.
Influenza viruses and mRNA splicing: doing more with less.
Dubois J, Terrier O, Rosa-Calatrava M mBio. 2014; 5(3):e00070-14.
PMID: 24825008 PMC: 4030477. DOI: 10.1128/mBio.00070-14.