Tissue Expression Pattern of ABCG Transporter Indicates Functional Roles in Reproduction of Toxocara Canis

Overview

Journal Parasitol Res

Specialty Parasitology

Date 2018 Feb 10

PMID 29423531

Citations 2

Authors

Yong-Li Luo

Guang-Xu Ma

Yong-Fang Luo

Ce-Yan Kuang

Ai-Yun Jiang

Guo-Qing Li

Rong-Qiong Zhou

Affiliations

Soon will be listed here.

Abstract

Toxocara canis is a zoonotic parasite with worldwide distribution. ATP-binding cassette (ABC) transporters are integral membrane proteins which involve in a range of biological processes in various organisms. In present study, the full-length coding sequence of abcg-5 gene of T. canis (Tc-abcg-5) was cloned and characterized. A 633 aa polypeptide containing two conserved Walker A and Walker B motifs was predicted from a continuous 1902 nt open reading frame. Quantitative real-time PCR was employed to determine the transcriptional levels of Tc-abcg-5 gene in adult male and female worms, which indicated high mRNA level of Tc-abcg-5 in the reproductive tract of adult female T. canis. Tc-abcg-5 was expressed to produce rabbit polyclonal antiserum against recombinant TcABCG5. Indirect-fluorescence immunohistochemical assays were carried out to detect the tissue distribution of TcABCG5, which showed predominant distribution of TcABCG5 in the uterus (especially in the germ cells) of adult female T. canis. Tissue transcription and expression pattern of Tc-abcg-5 indicated that Tc-abcg-5 might play essential roles in the reproduction of this parasitic nematode.

Citing Articles

Importance of ABC Transporters in the Survival of Parasitic Nematodes and the Prospect for the Development of Novel Control Strategies.

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Mechanics and pharmacology of substrate selection and transport by eukaryotic ABC exporters.

Srikant S, Gaudet R Nat Struct Mol Biol. 2019; 26(9):792-801.

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