Glycoprotein Ib Beta is the Only Phosphorylated Major Membrane Glycoprotein in Human Platelets

Overview

Journal Biochem J

Specialty Biochemistry

Date 1986 Mar 1

PMID 2941010

Citations 3

Authors

B Wyler

D Bienz

K J Clemetson

E F LUSCHER

Affiliations

Soon will be listed here.

Abstract

Platelets were metabolically labelled with 32P and the phosphoproteins examined by two-dimensional non-reduced/reduced gel electrophoresis and isoelectric-focusing/gel electrophoresis. Comparison with similar separations of surface-labelled platelets showed that the only major glycoprotein which is phosphorylated is the beta-subunit of glycoprotein Ib, indicating that this subunit contains a cytoplasmic segment. The identification was confirmed using immunoblotting with an antibody to the beta-subunit. Phosphoserine was the principal phosphorylation site, with some phosphothreonine, but phosphotyrosine was absent. No quantitative or qualitative differences could be detected in the phosphorylation of glycoprotein Ib beta from resting or activated platelets. These results exclude changes in phosphorylation of the major platelet membrane glycoproteins as a method of signal transmission by these receptors.

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