Simultaneous Determination of Four Sudan Dyes in Rat Blood by UFLC-MS/MS and Its Application to a Pharmacokinetic Study in Rats

Overview

Journal J Pharm Anal

Specialty Chemistry

Date 2018 Feb 7

PMID 29403937

Citations 1

Authors

Hao Zhu

Yijun Chen

Changshun Huang

Yangyang Han

Yiwei Zhang

Shucan Xie

Xiaohong Chen

Micong Jin

Affiliations

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Abstract

A rapid and sensitive method based on ultrafast liquid chromatography-tandem mass spectrometry was developed and validated for simultaneous determination of Sudan I, Sudan II, Sudan III, and Sudan IV levels in rat whole blood. Cleanert C18 mixed-mode polymeric sorbent was used for effective solid-phase extraction cleanup. Separation was carried out on a reversed-phase C column (100 mm×2.1 mm, 1.8 μm) using 0.1% (v/v) formic acid in water/0.1% (v/v) formic acid in acetonitrile as the mobile phase in gradient elution. Quantification was performed by an electrospray ionization source in the positive multiple reaction monitoring mode using D-Sudan I as the internal standard. Calibration curves showed good linearity between 0.2 and 20.0 μg/L, with correlation coefficients higher than 0.9990. The average recovery rates were between 93.05% and 114.98%. The intra- and inter-day relative standard deviations were within 6.2%. The lower limit of quantification was 0.2 μg/L. All the analytes were found to be stable in a series of stability studies. The proposed method was successfully applied to a pharmacokinetic study of four Sudan dyes after oral administration to rats.

Citing Articles

Voltammetric Determination of Sudan 1 in Food Samples Using Its Cu(II) Compound.

Karaboduk K, Hasdemir E Food Technol Biotechnol. 2019; 56(4):573-580.

PMID: 30923454 PMC: 6399716. DOI: 10.17113/ftb.56.04.18.5679.

References

Zhang Y, Zhang Z, Sun Y . Development and optimization of an analytical method for the determination of Sudan dyes in hot chilli pepper by high-performance liquid chromatography with on-line electrogenerated BrO- -luminol chemiluminescence detection. J Chromatogr A. 2006; 1129(1):34-40. DOI: 10.1016/j.chroma.2006.06.028. View

Ming L, Xi X, Chen T, Liu J . Electrochemical Determination of Trace Sudan I Contamination in Chili Powder at Carbon Nanotube Modified Electrodes. Sensors (Basel). 2016; 8(3):1890-1900. PMC: 3663031. DOI: 10.3390/s8031890. View

Schummer C, Sassel J, Bonenberger P, Moris G . Low-level detections of Sudan I, II, III and IV in spices and Chili-containing foodstuffs using UPLC-ESI-MS/MS. J Agric Food Chem. 2013; 61(9):2284-9. DOI: 10.1021/jf400602a. View

Marchei E, Papaseit E, Garcia-Algar O, Farre M, Pacifici R, Pichini S . Determination of atomoxetine and its metabolites in conventional and non-conventional biological matrices by liquid chromatography-tandem mass spectrometry. J Pharm Biomed Anal. 2011; 60:26-31. DOI: 10.1016/j.jpba.2011.11.009. View

Leveques A, Actis-Goretta L, Rein M, Williamson G, Dionisi F, Giuffrida F . UPLC-MS/MS quantification of total hesperetin and hesperetin enantiomers in biological matrices. J Pharm Biomed Anal. 2011; 57:1-6. DOI: 10.1016/j.jpba.2011.08.031. View

Noguerol-Cal R, Lopez-Vilarino J, Fernandez-Martinez G, Barral-Losada L, Gonzalez-Rodriguez M . High-performance liquid chromatography analysis of ten dyes for control of safety of commercial articles. J Chromatogr A. 2007; 1179(2):152-60. DOI: 10.1016/j.chroma.2007.11.099. View

Calbiani F, Careri M, Elviri L, Mangia A, Pistara L, Zagnoni I . Development and in-house validation of a liquid chromatography-electrospray-tandem mass spectrometry method for the simultaneous determination of Sudan I, Sudan II, Sudan III and Sudan IV in hot chilli products. J Chromatogr A. 2004; 1042(1-2):123-30. DOI: 10.1016/j.chroma.2004.05.027. View

Calbiani F, Careri M, Elviri L, Mangia A, Zagnoni I . Accurate mass measurements for the confirmation of Sudan azo-dyes in hot chilli products by capillary liquid chromatography-electrospray tandem quadrupole orthogonal-acceleration time of flight mass spectrometry. J Chromatogr A. 2004; 1058(1-2):127-35. View

Luthi G, Blangy V, Eap C, Ansermot N . Buprenorphine and norbuprenorphine quantification in human plasma by simple protein precipitation and ultra-high performance liquid chromatography tandem mass spectrometry. J Pharm Biomed Anal. 2013; 77:1-8. DOI: 10.1016/j.jpba.2012.12.023. View

10.

Stiborova M, Martinek V, Rydlova H, Hodek P, Frei E . Sudan I is a potential carcinogen for humans: evidence for its metabolic activation and detoxication by human recombinant cytochrome P450 1A1 and liver microsomes. Cancer Res. 2002; 62(20):5678-84. View

11.

Wu H, Guo J, Chen S, Liu X, Zhou Y, Zhang X . Recent developments in qualitative and quantitative analysis of phytochemical constituents and their metabolites using liquid chromatography-mass spectrometry. J Pharm Biomed Anal. 2012; 72:267-91. DOI: 10.1016/j.jpba.2012.09.004. View

12.

Han D, Yu M, Knopp D, Niessner R, Wu M, Deng A . Development of a highly sensitive and specific enzyme-linked immunosorbent assay for detection of Sudan I in food samples. J Agric Food Chem. 2007; 55(16):6424-30. DOI: 10.1021/jf071005j. View

13.

Molder K, Kunnapas A, Herodes K, Leito I . "Fast peaks" in chromatograms of Sudan dyes. J Chromatogr A. 2007; 1160(1-2):227-34. DOI: 10.1016/j.chroma.2007.05.052. View

14.

Qi Y, Shan W, Liu Y, Zhang Y, Wang J . Production of the polyclonal antibody against Sudan 3 and Immunoassay of Sudan dyes in food samples. J Agric Food Chem. 2012; 60(9):2116-22. DOI: 10.1021/jf300026x. View

15.

Zacharis C, Kika F, Tzanavaras P, Rigas P, Kyranas E . Development and validation of a rapid HPLC method for the determination of five banned fat-soluble colorants in spices using a narrow-bore monolithic column. Talanta. 2011; 84(2):480-6. DOI: 10.1016/j.talanta.2011.01.043. View

16.

Coulet M, Leblanc J, Setzer R . Application of the margin of exposure (MoE) approach to substances in food that are genotoxic and carcinogenic: EXAMPLE 12: Sudan I (CAS No. 842-07-9). Food Chem Toxicol. 2010; 48 Suppl 1:S106-11. DOI: 10.1016/j.fct.2009.10.018. View

17.

Wang Y, Wei D, Yang H, Yang Y, Xing W, Li Y . Development of a highly sensitive and specific monoclonal antibody-based enzyme-linked immunosorbent assay (ELISA) for detection of Sudan I in food samples. Talanta. 2009; 77(5):1783-9. DOI: 10.1016/j.talanta.2008.10.016. View

18.

Fan Y, Chen M, Shentu C, El-Sepai F, Wang K, Zhu Y . Ionic liquids extraction of Para Red and Sudan dyes from chilli powder, chilli oil and food additive combined with high performance liquid chromatography. Anal Chim Acta. 2009; 650(1):65-9. DOI: 10.1016/j.aca.2009.03.025. View

19.

Ahlstrom L, Bjorklund E, Mathiasson L . Optimization of an analytical procedure for the determination of banned azo dyes in leather. Anal Bioanal Chem. 2005; 382(5):1320-7. DOI: 10.1007/s00216-005-3240-2. View

20.

Matuszewski B, Constanzer M, Chavez-Eng C . Strategies for the assessment of matrix effect in quantitative bioanalytical methods based on HPLC-MS/MS. Anal Chem. 2003; 75(13):3019-30. DOI: 10.1021/ac020361s. View