MicroRNA-378 Regulates Cell Proliferation and Migration by Repressing RNF31 in Pituitary Adenoma

Overview

Journal Oncol Lett

Specialty Oncology

Date 2018 Feb 6

PMID 29399147

Citations 11

Authors

Peng Qiu

Tong-Jiang Xu

Xiang-Dong Lu

Wei Yang

Yu-Bao Zhang

Guang-Ming Xu

Affiliations

Soon will be listed here.

Abstract

MicroRNA-378 (miR-378) is dysregulated in multiple malignancies and is associated with tumor progression. However, the expression and mechanism of miR-378 in pituitary adenoma (PA) remains to be elucidated. In the present study, the role and mechanism of miR-378 in PA tumorigenesis and development was investigated. It was revealed that the levels of miR-378 expression were markedly downregulated in PA tissues. CCK-8 and wound healing assays revealed that transfection with miR-378 mimics was able to markedly inhibit the proliferation and migration of GH3 cells. Furthermore, quantitative polymerase chain reaction analysis demonstrated that ring finger protein 31 (RNF31) was upregulated in PA specimens and the levels of RNF31 expression was negatively regulated by miR-378. In addition, knockdown of RNF31 markedly suppressed cell proliferation and migration in GH3 cells. In conclusion, the present study provides a molecular basis for the function of miR-378/RNF31 in the progression of human PA, indicating a potential novel target for the treatment of PA.

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