The Use of Novel Epitope-tagged Arenaviruses Reveals That Rab5c-positive Endosomal Membranes Are Targeted by the LCMV Matrix Protein

Overview

Journal J Gen Virol

Specialty Microbiology

Date 2018 Feb 3

PMID 29393022

Citations 3

Authors

Christopher M Ziegler

Emily A Bruce

Jamie A Kelly

Benjamin R King

Jason W Botten

Affiliations

Soon will be listed here.

Abstract

We report the development of recombinant New World (Junín; JUNV) and Old World (lymphocytic choriomeningitis virus; LCMV) mammarenaviruses that encode an HA-tagged matrix protein (Z). These viruses permit the robust affinity purification of Z from infected cells or virions, as well as the detection of Z by immunofluorescent microscopy. Importantly, the HA-tagged viruses grow with wild-type kinetics in a multi-cycle growth assay. Using these viruses, we report a novel description of JUNV Z localization in infected cells, as well as the first description of colocalization between LCMV Z and the GTPase Rab5c. This latter result, when combined with our previous findings that LCMV genome and glycoprotein also colocalize with Rab5c, suggest that LCMV may target Rab5c-positive membranes for preassembly of virus particles prior to budding. The recombinant viruses reported here will provide the field with new tools to better study Z protein functionality and identify key Z protein interactions with host machinery.

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