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FlowerMorphology: Fully Automatic Flower Morphometry Software

Overview
Journal Planta
Specialty Biology
Date 2018 Feb 3
PMID 29392396
Citations 2
Authors
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Abstract

The software FlowerMorphology is designed for automatic morphometry of actinomorphic flowers. The novel complex parameters of flowers calculated by FlowerMorphology allowed us to quantitatively characterize a polyploid series of tobacco. Morphological differences of plants representing closely related lineages or mutants are mostly quantitative. Very often, there are only very fine variations in plant morphology. Therefore, accurate and high-throughput methods are needed for their quantification. In addition, new characteristics are necessary for reliable detection of subtle changes in morphology. FlowerMorphology is an all-in-one software package to automatically image and analyze five-petal actinomorphic flowers of the dicotyledonous plants. Sixteen directly measured parameters and ten calculated complex parameters of a flower allow us to characterize variations with high accuracy. The program was developed for the needs of automatic characterization of Nicotiana tabacum flowers, but is applicable to many other plants with five-petal actinomorphic flowers and can be adopted for flowers of other merosity. A genetically similar polyploid series of N. tabacum plants was used to investigate differences in flower morphology. For the first time, we could quantify the dependence between ploidy and size and form of the tobacco flowers. We found that the radius of inner petal incisions shows a persistent positive correlation with the chromosome number. In contrast, a commonly used parameter-radius of outer corolla-does not discriminate 2n and 4n plants. Other parameters show that polyploidy leads to significant aberrations in flower symmetry and are also positively correlated with chromosome number. Executables of FlowerMorphology, source code, documentation, and examples are available at the program website: https://github.com/Deyneko/FlowerMorphology .

Citing Articles

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Ballesta P, Maldonado C, Mora-Poblete F, Mieres-Castro D, Del Pozo A, Lobos G Plants (Basel). 2023; 12(3).

PMID: 36771526 PMC: 9920124. DOI: 10.3390/plants12030440.


Precision Phenotyping of Nectar-Related Traits Using X-ray Micro Computed Tomography.

Begot L, Slavkovic F, Oger M, Pichot C, Morin H, Boualem A Cells. 2022; 11(21).

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References
1.
Rapp R, Wendel J . Epigenetics and plant evolution. New Phytol. 2005; 168(1):81-91. DOI: 10.1111/j.1469-8137.2005.01491.x. View

2.
Kloster M, Kauer G, Beszteri B . SHERPA: an image segmentation and outline feature extraction tool for diatoms and other objects. BMC Bioinformatics. 2014; 15:218. PMC: 4087246. DOI: 10.1186/1471-2105-15-218. View

3.
Sidorchuk Y, Deineko E . Deformation of nuclei and abnormal spindles assembly in the second male meiosis of polyploid tobacco plants. Cell Biol Int. 2014; 38(4):472-9. DOI: 10.1002/cbin.10222. View

4.
Maliga P, Marton L . Streptomycin-resistant plants from callus culture of haploid tobacco. Nat New Biol. 1973; 244(131):29-30. DOI: 10.1038/newbio244029a0. View

5.
Deyneko I, Bredohl B, Wesely D, Kalybaeva Y, Kel A, Blocker H . FeatureScan: revealing property-dependent similarity of nucleotide sequences. Nucleic Acids Res. 2006; 34(Web Server issue):W591-5. PMC: 1538849. DOI: 10.1093/nar/gkl337. View