Protective Effect of Crocin Against D-galactose-induced Aging in Mice

Overview

Journal Avicenna J Phytomed

Publisher Mashhad University of Medical Sciences

Date 2018 Feb 2

PMID 29387570

Citations 14

Authors

Elaheh Mohammadi

Soghra Mehri

Hasan Badie Bostan

Hossein Hosseinzadeh

Affiliations

Soon will be listed here.

Abstract

Objective: Aging is a multifactorial phenomenon, which attribute to different diseases and abnormalities in living systems. Oxidative stress, which is an important factor in aging, exacerbates this process via different mechanisms. Crocin (CR), one of the active components of saffron showed strong antioxidant effects. In the present study, anti-aging property of crocin was investigated in mice.

Materials And Methods: The model of aging was induced using administration of d-galactose (500 mg/kg, s. c.) for 42 days. Animals were treated with crocin (10, 20, 40 mg/kg, i.p.) during treatment with d-galactose. At the end of treatment, levels of malondialdehyde (MDA) as a lipid peroxidation marker and glutathione content (GSH) in the liver and brain were measured. Also, biochemical factors including liver enzymes (ALT and AST), male sex hormones including testosterone and dehydroepiandrosterone-sulfate (DHEA-SO) and pro-inflammatory markers such as tumor necrosis factor -α (TNF-α) and interlukine-6 (IL-6) in serum, were evaluated.

Results: Administration of d-galactose led to induction of lipid peroxidation in liver and brain tissues, as well as elevation of AST, ALT, and pro-inflammatory cytokines and reduction of male sex hormones levels in serum. Interestingly, treatment of animals with crocin (10, 20 and 40 mg/kg) diminished lipid peroxidation in the liver and brain tissues while elevated GSH content. Also, a decline in serum levels of TNF-α and IL-6 and an elevation of male sex hormones were observed following treatment with crocin.

Conclusion: Administration of crocin reduced d-galactose-induced aging in mice through inhibition of oxidative stress, reduction of inflammation and elevation of sex hormones.

Citing Articles

Aging Parameters of the Accelerated Aging Procedure through D-Galactose Induction.

Kartika R, Timotius K, Sidharta V, Djuartina T, Sartika C Acta Med Philipp. 2025; 58(23):104-109.

PMID: 39830419 PMC: 11739527. DOI: 10.47895/amp.vi0.7801.

Radioprotective Effect of Resveratrol, Crocin, and Their Combination on Cytogenetic Alterations in Human Lymphocytes.

Faraji S, Moosavi S, Neshasteh-Riz A, Cheraghi S, Mayahi MSc S J Biomed Phys Eng. 2024; 14(3):255-266.

PMID: 39027705 PMC: 11252554. DOI: 10.31661/jbpe.v0i0.2109-1409.

Crocin's effect on phenotype switching of J774A.1 macrophages depends on their polarization state during exposure.

Abdi H, Roshanravan M, Mirzavi F, Hosseinzadeh H, Mosaffa F Iran J Basic Med Sci. 2023; 26(12):1431-1437.

PMID: 37970445 PMC: 10634050. DOI: 10.22038/IJBMS.2023.70859.15392.

Advances on the anti-tumor mechanisms of the carotenoid Crocin.

Bao X, Hu J, Zhao Y, Jia R, Zhang H, Xia L PeerJ. 2023; 11:e15535.

PMID: 37404473 PMC: 10315134. DOI: 10.7717/peerj.15535.

Protective Effects of Crocin Against Methotrexate-Induced Hepatotoxicity in Adult Male Albino Rats: Histological, Immunohistochemical, and Biochemical Study.

AbdelKader G, Abdelaziz E, Hassan R, Greish S, Abogresha N, Sultan B Cureus. 2023; 15(1):e34468.

PMID: 36874671 PMC: 9981239. DOI: 10.7759/cureus.34468.

References

Hsieh H, Wu W, Hu M . Soy isoflavones attenuate oxidative stress and improve parameters related to aging and Alzheimer's disease in C57BL/6J mice treated with D-galactose. Food Chem Toxicol. 2009; 47(3):625-32. DOI: 10.1016/j.fct.2008.12.026. View

Mehri S, Abnous K, Khooei A, Mousavi S, Shariaty V, Hosseinzadeh H . Crocin reduced acrylamide-induced neurotoxicity in Wistar rat through inhibition of oxidative stress. Iran J Basic Med Sci. 2015; 18(9):902-8. PMC: 4620190. View

Razavi B, Hosseinzadeh H, Movassaghi A, Imenshahidi M, Abnous K . Protective effect of crocin on diazinon induced cardiotoxicity in rats in subchronic exposure. Chem Biol Interact. 2013; 203(3):547-55. DOI: 10.1016/j.cbi.2013.03.010. View

Zheng Y, Liu J, Wang J, Xu L . Effects of crocin on reperfusion-induced oxidative/nitrative injury to cerebral microvessels after global cerebral ischemia. Brain Res. 2007; 1138:86-94. DOI: 10.1016/j.brainres.2006.12.064. View

Ruan Q, Liu F, Gao Z, Kong D, Hu X, Shi D . The anti-inflamm-aging and hepatoprotective effects of huperzine A in D-galactose-treated rats. Mech Ageing Dev. 2013; 134(3-4):89-97. DOI: 10.1016/j.mad.2012.12.005. View

Nam K, Park Y, Jung H, Lee J, Min B, Park S . Anti-inflammatory effects of crocin and crocetin in rat brain microglial cells. Eur J Pharmacol. 2010; 648(1-3):110-6. DOI: 10.1016/j.ejphar.2010.09.003. View

Hosseinzadeh H, Sadeghi Shakib S, Khadem Sameni A, Taghiabadi E . Acute and subacute toxicity of safranal, a constituent of saffron, in mice and rats. Iran J Pharm Res. 2013; 12(1):93-9. PMC: 3813202. View

Shamsa A, Hosseinzadeh H, Molaei M, Shakeri M, Rajabi O . Evaluation of Crocus sativus L. (saffron) on male erectile dysfunction: a pilot study. Phytomedicine. 2009; 16(8):690-3. DOI: 10.1016/j.phymed.2009.03.008. View

Hosseinzadeh H, Abootorabi A, Sadeghnia H . Protective effect of Crocus sativus stigma extract and crocin (trans-crocin 4) on methyl methanesulfonate-induced DNA damage in mice organs. DNA Cell Biol. 2008; 27(12):657-64. DOI: 10.1089/dna.2008.0767. View

10.

Mollazadeh H, Emami S, Hosseinzadeh H . Razi's Al-Hawi and saffron (Crocus sativus): a review. Iran J Basic Med Sci. 2016; 18(12):1153-66. PMC: 4744354. View

11.

Mihara M, Uchiyama M . Determination of malonaldehyde precursor in tissues by thiobarbituric acid test. Anal Biochem. 1978; 86(1):271-8. DOI: 10.1016/0003-2697(78)90342-1. View

12.

Kumar A, Prakash A, Dogra S . Centella asiatica Attenuates D-Galactose-Induced Cognitive Impairment, Oxidative and Mitochondrial Dysfunction in Mice. Int J Alzheimers Dis. 2011; 2011:347569. PMC: 3100561. DOI: 10.4061/2011/347569. View

13.

Haider S, Liaquat L, Shahzad S, Sadir S, Madiha S, Batool Z . A high dose of short term exogenous D-galactose administration in young male rats produces symptoms simulating the natural aging process. Life Sci. 2015; 124:110-9. DOI: 10.1016/j.lfs.2015.01.016. View

14.

Li K, Li Y, Ma Z, Zhao J . Crocin exerts anti-inflammatory and anti-catabolic effects on rat intervertebral discs by suppressing the activation of JNK. Int J Mol Med. 2015; 36(5):1291-9. PMC: 4601741. DOI: 10.3892/ijmm.2015.2359. View

15.

Zhang Z, Fan S, Zheng Y, Lu J, Wu D, Shan Q . Troxerutin protects the mouse liver against oxidative stress-mediated injury induced by D-galactose. J Agric Food Chem. 2009; 57(17):7731-6. DOI: 10.1021/jf9012357. View

16.

Jauslin M, Vertuani S, Durini E, Buzzoni L, Ciliberti N, Verdecchia S . Protective effects of Fe-Aox29, a novel antioxidant derived from a molecular combination of Idebenone and vitamin E, in immortalized fibroblasts and fibroblasts from patients with Friedreich Ataxia. Mol Cell Biochem. 2007; 302(1-2):79-85. DOI: 10.1007/s11010-007-9429-2. View

17.

Zhong S, Ge Q, Qu R, Li Q, Ma S . Paeonol attenuates neurotoxicity and ameliorates cognitive impairment induced by d-galactose in ICR mice. J Neurol Sci. 2008; 277(1-2):58-64. DOI: 10.1016/j.jns.2008.10.008. View

18.

Alavizadeh S, Hosseinzadeh H . Bioactivity assessment and toxicity of crocin: a comprehensive review. Food Chem Toxicol. 2013; 64:65-80. DOI: 10.1016/j.fct.2013.11.016. View

19.

Lan Z, Liu J, Chen L, Fu Q, Luo J, Qu R . Danggui-Shaoyao-San ameliorates cognition deficits and attenuates oxidative stress-related neuronal apoptosis in d-galactose-induced senescent mice. J Ethnopharmacol. 2012; 141(1):386-95. DOI: 10.1016/j.jep.2012.02.050. View

20.

Wang D, Liu M, Cao J, Cheng Y, Zhuo C, Xu H . Effect of Colla corii asini (E'jiao) on D-galactose induced aging mice. Biol Pharm Bull. 2012; 35(12):2128-32. DOI: 10.1248/bpb.b12-00238. View