Over-expression of Suppressed Cell Proliferation, Migration, Invasion, and Tumor Growth in Gastric Cancer by Restraining Over-activation of MAPK Signaling Pathways

Overview

Journal Onco Targets Ther

Publisher Dove Medical Press

Specialty Oncology

Date 2018 Feb 2

PMID 29386906

Citations 7

Authors

Yan Li

Shan Ji

Liye Fu

Tao Jiang

Di Wu

Fandong Meng

Affiliations

Soon will be listed here.

Abstract

Globally, gastric cancer is the second-greatest cause of cancer death. belongs to the Rho family of GTPases which is involved in cellular migration, invasion, and growth phases. The aim of the present study was to investigate whether could regulate cell proliferation, migration, invasion, and related molecular mechanisms in gastric cancer. Cell Counting Kit-8 (CCK-8) assay results showed that following transfection of a recombinant plasmid, over-expression of inhibited cell viability in MGC-803 and BGC823 cells. Using in vitro transwell analysis, migration and invasion abilities were significantly inhibited in cells with high expression. Phosphorylation levels of ERK, JNK, and p38 by Western blot analysis significantly declined after transfection of cells with the plasmid. Expression levels of ROCK, MTA1, and MMP-2/9 were detected by real-time polymerase chain reaction and Western blotting, and over-expression of decreased the expression levels of ROCK, MTA1, and MMP-9. A further in vivo tumor formation study in nude mice indicated that over-expression of delayed the progress of tumor formation. These results indicate that could act as a tumor suppressor and may serve as a promising therapeutic strategy for gastric cancer.

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