TelAP1 Links Telomere Complexes with Developmental Expression Site Silencing in African Trypanosomes

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 2018 Feb 1

PMID 29385523

Citations 18

Authors

Helena Reis

Marie Schwebs

Sabrina Dietz

Christian J Janzen

Falk Butter

Affiliations

Soon will be listed here.

Abstract

During its life cycle, Trypanosoma brucei shuttles between a mammalian host and the tsetse fly vector. In the mammalian host, immune evasion of T. brucei bloodstream form (BSF) cells relies on antigenic variation, which includes monoallelic expression and periodic switching of variant surface glycoprotein (VSG) genes. The active VSG is transcribed from only 1 of the 15 subtelomeric expression sites (ESs). During differentiation from BSF to the insect-resident procyclic form (PCF), the active ES is transcriptionally silenced. We used mass spectrometry-based interactomics to determine the composition of telomere protein complexes in T. brucei BSF and PCF stages to learn more about the structure and functions of telomeres in trypanosomes. Our data suggest a different telomere complex composition in the two forms of the parasite. One of the novel telomere-associated proteins, TelAP1, forms a complex with telomeric proteins TbTRF, TbRAP1 and TbTIF2 and influences ES silencing kinetics during developmental differentiation.

Citing Articles

TelAP2 links TelAP1 to the telomere complex in Trypanosoma brucei.

Weisert N, Majewski V, Hartleb L, Luko K, Lototska L, Krapoth N Sci Rep. 2024; 14(1):30493.

PMID: 39681615 PMC: 11649696. DOI: 10.1038/s41598-024-81972-0.

Unwrap RAP1's Mystery at Kinetoplastid Telomeres.

Li B Biomolecules. 2024; 14(1).

PMID: 38254667 PMC: 10813129. DOI: 10.3390/biom14010067.

An allele-selective inter-chromosomal protein bridge supports monogenic antigen expression in the African trypanosome.

Faria J, Tinti M, Marques C, Zoltner M, Yoshikawa H, Field M Nat Commun. 2023; 14(1):8200.

PMID: 38081826 PMC: 10713589. DOI: 10.1038/s41467-023-44043-y.

Telomere maintenance in African trypanosomes.

Li B Front Mol Biosci. 2023; 10:1302557.

PMID: 38074093 PMC: 10704157. DOI: 10.3389/fmolb.2023.1302557.

The developmental hierarchy and scarcity of replicative slender trypanosomes in blood challenges their role in infection maintenance.

Larcombe S, Briggs E, Savill N, Szoor B, Matthews K Proc Natl Acad Sci U S A. 2023; 120(42):e2306848120.

PMID: 37824530 PMC: 10589647. DOI: 10.1073/pnas.2306848120.

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