Functional Characterization of a Unique Cytochrome P450 in

Overview

Journal Oncotarget

Specialty Oncology

Date 2018 Feb 1

PMID 29383143

Citations 4

Authors

Xiao Zhang

Taotao Zhang

Jing Liu

Muzi Li

Yong Fu

Jianhai Xu

Qun Liu

Affiliations

Soon will be listed here.

Abstract

The basic metabolic cytochrome P450 (CYP) proteins are essential for the biotransformation of sterols and xenobiotics. By contrast, the genome contains only one CYP gene, and the role of this enzyme in the physiology and biochemistry of apicomplexan parasites is unknown. Because it is a potential resistance gene, identifying the functionality of P450 in is particularly important. Knocking out had no significant effect on survival, but mice infected with parasites overexpressing exhibited significantly enhanced pathogenicity. Enzyme activity analyses demonstrated that this protein has mammalian CYP2B and CYP3A enzymatic activity. In addition, lacking the P450 gene exhibited reduced resistance to quinine, mefloquine and clarithromycin compared with parasites overexpressing Tg-P450. These results suggest that P450 functions in metabolism and detoxification is involved in vitally important processes in parasitic organisms, making this enzyme a potential drug target.

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