Activity of Chi Recombinational Hotspots in Salmonella Typhimurium

Overview

Journal Genetics

Publisher Oxford University Press

Specialty Genetics

Date 1986 Mar 1

PMID 2937685

Citations 15

Authors

G R Smith

C M Roberts

D W Schultz

Affiliations

Soon will be listed here.

Abstract

Chi sites have previously been shown to stimulate homologous recombination by the Escherichia coli RecBC pathway. To test the activity of Chi in another organism, bacteriophage lambda crosses were carried out in Salmonella typhimurium strains bearing the E. coli lambda receptor protein. Chi is active in these crosses in S. typhimurium, but is less active than in the same crosses carried out in E. coli. The lower Chi activity in S. typhimurium appears to be intrinsic to the S. typhimurium RecBC enzyme, since the Chi activity in E. coli-S. typhimurium hybrids depends on the species of origin of their RecBC enzyme. For these studies we constructed and F' factor and a pBR322-derived plasmid carrying the thyA+ recC+ recB+ argA+ region of the S. typhimurium chromosome.

Citing Articles

A flexible RecC surface loop required for Chi hotspot control of RecBCD enzyme.

Amundsen S, Richardson A, Ha K, Smith G Genetics. 2022; 223(3).

PMID: 36521180 PMC: 9991510. DOI: 10.1093/genetics/iyac175.

RecBCD enzyme and Chi recombination hotspots as determinants of self vs. non-self: Myths and mechanisms.

Subramaniam S, Smith G Adv Genet. 2022; 109:1-37.

PMID: 36334915 PMC: 10047805. DOI: 10.1016/bs.adgen.2022.06.001.

Gene essentiality profiling reveals a novel determinant of stresses preventing protein aggregation in .

Wang Z, Zhu S, Li C, Lyu L, Yu J, Wang D Emerg Microbes Infect. 2022; 11(1):1554-1571.

PMID: 35603550 PMC: 9176671. DOI: 10.1080/22221751.2022.2081618.

Evolution of Chi motifs in Proteobacteria.

Buton A, Bobay L G3 (Bethesda). 2021; 11(1).

PMID: 33561247 PMC: 8022716. DOI: 10.1093/g3journal/jkaa054.

RecBCD Enzyme "Chi Recognition" Mutants Recognize Chi Recombination Hotspots in the Right DNA Context.

Amundsen S, Sharp J, Smith G Genetics. 2016; 204(1):139-52.

PMID: 27401752 PMC: 5012381. DOI: 10.1534/genetics.116.191056.

References

Smith G, Kunes S, Schultz D, Taylor A, Triman K . Structure of chi hotspots of generalized recombination. Cell. 1981; 24(2):429-36. DOI: 10.1016/0092-8674(81)90333-0. View

Kenter A, Birshtein B . Chi, a promoter of generalized recombination in lambda phage, is present in immunoglobulin genes. Nature. 1981; 293(5831):402-4. DOI: 10.1038/293402a0. View

Sasaki M, Fujiyoshi T, Shimada K, Takagi Y . Fine structure of the recB and recC gene region of Escherichia coli. Biochem Biophys Res Commun. 1982; 109(2):414-22. DOI: 10.1016/0006-291x(82)91737-5. View

SANGER F, Coulson A, Hong G, Hill D, Petersen G . Nucleotide sequence of bacteriophage lambda DNA. J Mol Biol. 1982; 162(4):729-73. DOI: 10.1016/0022-2836(82)90546-0. View

Schultz D, Taylor A, Smith G . Escherichia coli RecBC pseudorevertants lacking chi recombinational hotspot activity. J Bacteriol. 1983; 155(2):664-80. PMC: 217737. DOI: 10.1128/jb.155.2.664-680.1983. View

Kobayashi I, Murialdo H, Crasemann J, Stahl M, Stahl F . Orientation of cohesive end site cos determines the active orientation of chi sequence in stimulating recA . recBC-mediated recombination in phage lambda lytic infections. Proc Natl Acad Sci U S A. 1982; 79(19):5981-5. PMC: 347035. DOI: 10.1073/pnas.79.19.5981. View

Smith G, Comb M, Schultz D, Daniels D, Blattner F . Nucleotide sequence of the chi recombinational hot spot chi +D in bacteriophage lambda. J Virol. 1981; 37(1):336-42. PMC: 171011. DOI: 10.1128/JVI.37.1.336-342.1981. View

Chaudhury A, Smith G . A new class of Escherichia coli recBC mutants: implications for the role of RecBC enzyme in homologous recombination. Proc Natl Acad Sci U S A. 1984; 81(24):7850-4. PMC: 392250. DOI: 10.1073/pnas.81.24.7850. View

Mandel M, Higa A . Calcium-dependent bacteriophage DNA infection. J Mol Biol. 1970; 53(1):159-62. DOI: 10.1016/0022-2836(70)90051-3. View

10.

Sanderson K, Ross H, Ziegler L, Makela P . F + , Hfr, and F' strains of Salmonella typhimurium and Salmonella abony. Bacteriol Rev. 1972; 36(4):608-37. PMC: 408334. DOI: 10.1128/br.36.4.608-637.1972. View

11.

Friedman D, Baron L . Genetic characterization of a bacterial locus involved in the activity of the N function of phage lambda. Virology. 1974; 58(1):141-8. DOI: 10.1016/0042-6822(74)90149-4. View

12.

Henderson D, Weil J . Recombination-deficient deletions in bacteriophage lambda and their interaction with chi mutations. Genetics. 1975; 79(2):143-74. PMC: 1213264. DOI: 10.1093/genetics/79.2.143. View

13.

Chattoraj D, Crasemann J, Dower N, Faulds D, Faulds P, Malone R . Chi. Cold Spring Harb Symp Quant Biol. 1979; 43 Pt 2:1063-6. DOI: 10.1101/sqb.1979.043.01.116. View

14.

Lundblad V, Taylor A, Smith G, Kleckner N . Unusual alleles of recB and recC stimulate excision of inverted repeat transposons Tn10 and Tn5. Proc Natl Acad Sci U S A. 1984; 81(3):824-8. PMC: 344930. DOI: 10.1073/pnas.81.3.824. View