Platycodin D Inhibits Inflammatory Response in LPS-Stimulated Primary Rat Microglia Cells Through Activating LXRα-ABCA1 Signaling Pathway

Overview

Journal Front Immunol

Specialty Allergy & Immunology

Date 2018 Jan 30

PMID 29375565

Citations 20

Authors

Yunhe Fu

Zhuoyuan Xin

Bin Liu

Jiaxin Wang

Jingjing Wang

Xu Zhang

Yanan Wang

Fan Li

Affiliations

Soon will be listed here.

Abstract

Platycodin D (PLD), an effective triterpenesaponin extracted from , has been known to have anti-inflammatory effect. In the present study, we investigate the anti-inflammatory effects of PLD on LPS-induced inflammation in primary rat microglia cells. The results showed that PLD significantly inhibited LPS-induced ROS, TNF-α, IL-6, and IL-1β production in primary rat microglia cells. PLD also inhibited LPS-induced NF-κB activation. Furthermore, our results showed that PLD prevented LPS-induced TLR4 translocation into lipid rafts disrupting the formation of lipid rafts by inducing cholesterol efflux. In addition, PLD could activate LXRα-ABCA1 signaling pathway which induces cholesterol efflux from cells. The inhibition of inflammatory cytokines by PLD could be reversed by SiRNA of LXRα. In conclusion, these results indicated that PLD prevented LPS-induced inflammation by activating LXRα-ABCA1 signaling pathway, which disrupted lipid rafts and prevented TLR4 translocation into lipid rafts, thereby inhibiting LPS-induced inflammatory response.

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