Overcoming Antimicrobial Resistance in Bacteria Using Bioactive Magnetic Nanoparticles and Pulsed Electromagnetic Fields

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2018 Jan 30

PMID 29375537

Citations 12

Authors

Vitalij Novickij

Ramune Staneviciene

Igle Vepstaite-Monstavice

Ruta Gruskiene

Tatjana Krivorotova

Jolanta Sereikaite

Jurij Novickij

Elena Serviene

Affiliations

Soon will be listed here.

Abstract

Nisin is a known bacteriocin, which exhibits a wide spectrum of antimicrobial activity, while commonly being inefficient against Gram-negative bacteria. In this work, we present a proof of concept of novel antimicrobial methodology using targeted magnetic nisin-loaded nano-carriers [iron oxide nanoparticles (NPs) (11-13 nm) capped with citric, ascorbic, and gallic acids], which are activated by high pulsed electric and electromagnetic fields allowing to overcome the nisin-resistance of bacteria. As a cell model the Gram-positive bacteria and Gram-negative were used. We have applied 10 and 30 kV cm electric field pulses (100 μs × 8) separately and in combination with two pulsed magnetic field protocols: (1) high d/d 3.3 T × 50 and (2) 10 mT, 100 kHz, 2 min protocol to induce additional permeabilization and local magnetic hyperthermia. We have shown that the high d/d pulsed magnetic fields increase the antimicrobial efficiency of nisin NPs similar to electroporation or magnetic hyperthermia methods and a synergistic treatment is also possible. The results of our work are promising for the development of new methods for treatment of the drug-resistant foodborne pathogens to minimize the risks of invasive infections.

Citing Articles

Microbiome modulation of implant-related infection by a novel miniaturized pulsed electromagnetic field device.

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A Double-Blinded Randomized Controlled Trial: Can Pulsed Electromagnetic Field Therapy Be a Novel Method for Treating Chronic Rhinosinusitis?.

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High-Intensity Pulsed Electromagnetic Field-Mediated Gene Electrotransfection In Vitro.

Kranjc M, Dermol-Cerne J, Potocnik T, Novickij V, Miklavcic D Int J Mol Sci. 2022; 23(17).

PMID: 36076938 PMC: 9455820. DOI: 10.3390/ijms23179543.

Effect of pine essential oil and rotating magnetic field on antimicrobial performance.

Markowska-Szczupak A, Wesolowska A, Borowski T, Soloducha D, Paszkiewicz O, Kordas M Sci Rep. 2022; 12(1):9712.

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The effects of rotating magnetic field and antiseptic on in vitro pathogenic biofilm and its milieu.

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