Label-Free Aptasensor for Lysozyme Detection Using Electrochemical Impedance Spectroscopy

Overview

Journal Sensors (Basel)

Publisher MDPI

Specialty Biotechnology

Date 2018 Jan 27

PMID 29373502

Citations 13

Authors

Dionisia Ortiz-Aguayo

Manel Del Valle

Affiliations

Soon will be listed here.

Abstract

This research develops a label-free aptamer biosensor (aptasensor) based on graphite-epoxy composite electrodes (GECs) for the detection of lysozyme protein using Electrochemical Impedance Spectroscopy (EIS) technique. The chosen immobilization technique was based on covalent bonding using carbodiimide chemistry; for this purpose, carboxylic moieties were first generated on the graphite by electrochemical grafting. The detection was performed using [Fe(CN)₆]/[Fe(CN)₆] as redox probe. After recording the frequency response, values were fitted to its electric model using the principle of equivalent circuits. The aptasensor showed a linear response up to 5 µM for lysozyme and a limit of detection of 1.67 µM. The sensitivity of the established method was 0.090 µM in relative charge transfer resistance values. The interference response by main proteins, such as bovine serum albumin and cytochrome c, has been also characterized. To finally verify the performance of the developed aptasensor, it was applied to wine analysis.

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References

Thevenot D, Toth K, Durst R, Wilson G . Electrochemical biosensors: recommended definitions and classification. Biosens Bioelectron. 2001; 16(1-2):121-31. DOI: 10.1016/s0956-5663(01)00115-4. View

Bonanni A, Pividori M, Del Valle M . Application of the avidin-biotin interaction to immobilize DNA in the development of electrochemical impedance genosensors. Anal Bioanal Chem. 2007; 389(3):851-61. DOI: 10.1007/s00216-007-1490-x. View

Ocana C, Pacios M, Del Valle M . A reusable impedimetric aptasensor for detection of thrombin employing a graphite-epoxy composite electrode. Sensors (Basel). 2012; 12(3):3037-48. PMC: 3376577. DOI: 10.3390/s120303037. View

Lisdat F, Schafer D . The use of electrochemical impedance spectroscopy for biosensing. Anal Bioanal Chem. 2008; 391(5):1555-67. DOI: 10.1007/s00216-008-1970-7. View

Nimjee S, Rusconi C, Sullenger B . Aptamers: an emerging class of therapeutics. Annu Rev Med. 2005; 56:555-83. DOI: 10.1146/annurev.med.56.062904.144915. View

Vasilescu A, Gaspar S, Mihai I, Tache A, Litescu S . Development of a label-free aptasensor for monitoring the self-association of lysozyme. Analyst. 2013; 138(12):3530-7. DOI: 10.1039/c3an00229b. View

Crivianu-Gaita V, Thompson M . Aptamers, antibody scFv, and antibody Fab' fragments: An overview and comparison of three of the most versatile biosensor biorecognition elements. Biosens Bioelectron. 2016; 85:32-45. DOI: 10.1016/j.bios.2016.04.091. View

Radi A, Sanchez J, Baldrich E, OSullivan C . Reusable impedimetric aptasensor. Anal Chem. 2005; 77(19):6320-3. DOI: 10.1021/ac0505775. View

Ocana C, Hayat A, Mishra R, Vasilescu A, Del Valle M, Marty J . Label free aptasensor for Lysozyme detection: A comparison of the analytical performance of two aptamers. Bioelectrochemistry. 2015; 105:72-7. DOI: 10.1016/j.bioelechem.2015.05.009. View

10.

Tombelli S, Minunni M, Mascini M . Analytical applications of aptamers. Biosens Bioelectron. 2005; 20(12):2424-34. DOI: 10.1016/j.bios.2004.11.006. View

11.

Porstmann B, Jung K, Schmechta H, Evers U, Pergande M, Porstmann T . Measurement of lysozyme in human body fluids: comparison of various enzyme immunoassay techniques and their diagnostic application. Clin Biochem. 1989; 22(5):349-55. DOI: 10.1016/s0009-9120(89)80031-1. View

12.

Bonanni A, Esplandiu M, Pividori M, Alegret S, Del Valle M . Impedimetric genosensors for the detection of DNA hybridization. Anal Bioanal Chem. 2006; 385(7):1195-201. DOI: 10.1007/s00216-006-0558-3. View

13.

Yang X, Qian J, Jiang L, Yan Y, Wang K, Liu Q . Ultrasensitive electrochemical aptasensor for ochratoxin A based on two-level cascaded signal amplification strategy. Bioelectrochemistry. 2013; 96:7-13. DOI: 10.1016/j.bioelechem.2013.11.006. View

14.

Belanger D, Pinson J . Electrografting: a powerful method for surface modification. Chem Soc Rev. 2011; 40(7):3995-4048. DOI: 10.1039/c0cs00149j. View

15.

Bonanni A, Del Valle M . Use of nanomaterials for impedimetric DNA sensors: a review. Anal Chim Acta. 2010; 678(1):7-17. DOI: 10.1016/j.aca.2010.08.022. View

16.

Pascual R, GEE J, Finch S . Usefulness of serum lysozyme measurement in diagnosis and evaluation of sarcoidosis. N Engl J Med. 1973; 289(20):1074-6. DOI: 10.1056/NEJM197311152892007. View

17.

Schindler M, Assaf Y, Sharon N, Chipman D . Mechanism of lysozyme catalysis: role of ground-state strain in subsite D in hen egg-white and human lysozymes. Biochemistry. 1977; 16(3):423-31. DOI: 10.1021/bi00622a013. View