Multilocus Sequence Typing Reveals Both Shared and Unique Genotypes of Cryptococcus Neoformans in Jiangxi Province, China

Overview

Journal Sci Rep

Specialty Science

Date 2018 Jan 26

PMID 29367679

Citations 20

Authors

Yan-Hui Chen

Feng Yu

Ze-Yuan Bian

Jian-Ming Hong

Nan Zhang

Qiao-Shi Zhong

Ya-Ping Hang

Jianping Xu

Long-Hua Hu

Affiliations

Soon will be listed here.

Abstract

Cryptococcosis is a globally distributed infectious fungal disease. However, much remains unknown about its molecular epidemiology in many parts of the world. In this study, we analyzed 86 clinical Cryptococcus neoformans isolates from 14 regions in Jiangxi Province in south central China. Each isolate was from a different patient and 35 of the 86 (40.7%) patients were infected with HIV. All strains belonged to serotype A and mating type α (MATα). Genotyping based on DNA sequences at seven nuclear loci revealed eight sequence types (STs) among the 86 isolates, including two novel STs that have not been reported from other parts of the world. ST5 was the dominant genotype and our comparative analyses showed that these genotypes in Jiangxi likely originated by dispersal from other regions within and outside of China and/or mutations from another genotype within Jiangxi. Though none of the isolates was resistant to the five tested antifungal drugs (flucytosine, amphotericin B, fluconazole, itraconazole, and voriconazole), obvious differences in their minimum inhibitory concentrations were observed, even among isolates of the same ST. Our results suggest that continuous monitoring should be conducted to understand the changing dynamics of C. neoformans in this and other regions.

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