Segregation of Central Ventricular Conduction System Lineages in Early SMA+ Cardiomyocytes Occurs Prior to Heart Tube Formation

Overview

Journal J Cardiovasc Dev Dis

Specialty Cardiology & Vascular Diseases

Date 2018 Jan 26

PMID 29367554

Citations 8

Authors

Caroline Choquet

Laetitia Marcadet

Sabrina Beyer

Robert G Kelly

Lucile Miquerol

Affiliations

Soon will be listed here.

Abstract

The cardiac conduction system (CCS) transmits electrical activity from the atria to the ventricles to coordinate heartbeats. Atrioventricular conduction diseases are often associated with defects in the central ventricular conduction system comprising the atrioventricular bundle (AVB) and right and left branches (BBs). Conducting and contractile working myocytes share common cardiomyogenic progenitors, however the time at which the CCS lineage becomes specified is unclear. In order to study the fate and the contribution to the CCS of cardiomyocytes during early heart tube formation, we performed a genetic lineage analysis using a mouse line. Lineage tracing experiments reveal a sequential contribution of early expressing cardiomyocytes to different cardiac compartments, labeling at embryonic day (E) 7.5 giving rise to the interventricular septum and apical left ventricular myocardium. Early expressing cardiomyocytes contribute to the AVB, BBs and left ventricular Purkinje fibers. Clonal analysis using the reporter mouse crossed with demonstrates that early expressing cardiomyocytes include cells exclusively fated to give rise to the AVB. In contrast, lineage segregation is still ongoing for the BBs at E7.5. Overall this study highlights the early segregation of the central ventricular conduction system lineage within cardiomyocytes at the onset of heart tube formation.

Citing Articles

Molecular Regulation of Cardiac Conduction System Development.

Boulgakoff L, DAmato G, Miquerol L Curr Cardiol Rep. 2024; 26(9):943-952.

PMID: 38990492 DOI: 10.1007/s11886-024-02094-7.

enhancer activity defines unipotent progenitors for left ventricular cardiomyocytes in juxta-cardiac field of early mouse embryo.

Watanabe Y, Wang Y, Tanaka Y, Iwase A, Kawamura T, Saga Y Proc Natl Acad Sci U S A. 2023; 120(37):e2307658120.

PMID: 37669370 PMC: 10500178. DOI: 10.1073/pnas.2307658120.

New Insights into the Development and Morphogenesis of the Cardiac Purkinje Fiber Network: Linking Architecture and Function.

Choquet C, Boulgakoff L, Kelly R, Miquerol L J Cardiovasc Dev Dis. 2021; 8(8).

PMID: 34436237 PMC: 8397066. DOI: 10.3390/jcdd8080095.

Unveiling Complexity and Multipotentiality of Early Heart Fields.

Zhang Q, Carlin D, Zhu F, Cattaneo P, Ideker T, Evans S Circ Res. 2021; 129(4):474-487.

PMID: 34162224 PMC: 9308985. DOI: 10.1161/CIRCRESAHA.121.318943.

Nkx2-5 defines distinct scaffold and recruitment phases during formation of the murine cardiac Purkinje fiber network.

Choquet C, Kelly R, Miquerol L Nat Commun. 2020; 11(1):5300.

PMID: 33082351 PMC: 7575572. DOI: 10.1038/s41467-020-19150-9.

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