Purification and Partial Characterization of Human Lymphoblast Interferon

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1979 Nov 1

PMID 293665

Citations 17

Authors

K C Zoon

M E Smith

P J Bridgen

D zur Nedden

C B ANFINSEN

Affiliations

Soon will be listed here.

Abstract

One component of human lymphoblastoid interferon obtained from Namalwa cultures induced by Newcastle disease virus has been purified to a specific activity of 2.5 x 10(8) interferon units per mg of protein (protein content based on amino acid analysis). A single polypeptide species with an apparent molecular weight of 18,500 comigrating with the antiviral activity was observed by sodium dodecyl sulfate/polyacrylamide gel electrophoresis. Preliminary amino-terminal sequencing results support the conclusion that the interferon species is essentially homogeneous.

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