Paeoniflorin, the Main Active Ingredient of Shuyu Capsule, Inhibits Ca1.2 and Regulates Calmodulin/Calmodulin-Dependent Protein Kinase II Signalling

Overview

Journal Biomed Res Int

Publisher Wiley

Specialties Biomedical Engineering
Biotechnology
General Medicine

Date 2018 Jan 25

PMID 29362718

Citations 5

Authors

Chunhong Song

Jieqiong Wang

Dongmei Gao

Yanhong Yu

Fang Li

Sheng Wei

Peng Sun

Meiyan Wang

Mingqi Qiao

Affiliations

Soon will be listed here.

Abstract

The aim of this study was to explore the mechanism underlying the antidepression activity of paeoniflorin, the main active ingredient of paeony extract and Shuyu capsules, and determine its effect on the calmodulin/calmodulin-dependent protein kinase II (CaM/CaMKII) signalling pathway and on the possible target, the voltage-gated calcium channel (Ca). Rats at the nonacceptance stage were selected for premenstrual syndrome (PMS) depression modelling. Behavioural assays were used for model testing. Rats were given Shuyu capsules, paeony extract, and bupleurum. Western blot analysis was used to assess the expression levels of calcium voltage-gated channel subunit alpha 1 C (CACNA1C), brain-derived neurotrophic factor, and CaM/CaMKII signalling pathway proteins. Intracellular Ca concentration in CHO cell line was measured using Fluo-4-AM and whole-cell patch clamps. The PMS depression model was successfully established and demonstrated that Shuyu can mitigate depressive behaviour in a rat PMS model. Paeony extract did not affect CACNA1C protein expression in rat hippocampi but did affect Ca1.2-mediated CaM/CaMKII signalling pathways. Paeoniflorin significantly inhibited KCl-induced increases in intracellular Ca concentration and Ca1.2 current density. Further, it may function via the CaM/CaMKII pathway and its downstream signalling molecules by regulating Ca1.2, thus playing an important role in the treatment and alleviation of affective disorders.

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