A Bioactive Hydrogel and 3D Printed Polycaprolactone System for Bone Tissue Engineering

Overview

Journal Gels

Date 2018 Jan 23

PMID 29354645

Citations 28

Authors

Ivan Hernandez

Alok Kumar

Binata Joddar

Affiliations

Soon will be listed here.

Abstract

In this study, a hybrid system consisting of 3D printed polycaprolactone (PCL) filled with hydrogel was developed as an application for reconstruction of long bone defects, which are innately difficult to repair due to large missing segments of bone. A 3D printed gyroid scaffold of PCL allowed a larger amount of hydrogel to be loaded within the scaffolds as compared to 3D printed mesh and honeycomb scaffolds of similar volumes and strut thicknesses. The hydrogel was a mixture of alginate, gelatin, and nano-hydroxyapatite, infiltrated with human mesenchymal stem cells (hMSC) to enhance the osteoconductivity and biocompatibility of the system. Adhesion and viability of hMSC in the PCL/hydrogel system confirmed its cytocompatibility. Biomineralization tests in simulated body fluid (SBF) showed the nucleation and growth of apatite crystals, which confirmed the bioactivity of the PCL/hydrogel system. Moreover, dissolution studies, in SBF revealed a sustained dissolution of the hydrogel with time. Overall, the present study provides a new approach in bone tissue engineering to repair bone defects with a bioactive hybrid system consisting of a polymeric scaffold, hydrogel, and hMSC.

Citing Articles

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References

Moreau J, Xu H . Mesenchymal stem cell proliferation and differentiation on an injectable calcium phosphate-chitosan composite scaffold. Biomaterials. 2009; 30(14):2675-82. PMC: 2662619. DOI: 10.1016/j.biomaterials.2009.01.022. View

Kuijpers A, Engbers G, Krijgsveld J, Zaat S, Dankert J, Feijen J . Cross-linking and characterisation of gelatin matrices for biomedical applications. J Biomater Sci Polym Ed. 2000; 11(3):225-43. DOI: 10.1163/156856200743670. View

Oyane A, Kim H, Furuya T, Kokubo T, Miyazaki T, Nakamura T . Preparation and assessment of revised simulated body fluids. J Biomed Mater Res A. 2003; 65(2):188-95. DOI: 10.1002/jbm.a.10482. View

Burastero G, Scarfi S, Ferraris C, Fresia C, Sessarego N, Fruscione F . The association of human mesenchymal stem cells with BMP-7 improves bone regeneration of critical-size segmental bone defects in athymic rats. Bone. 2010; 47(1):117-26. DOI: 10.1016/j.bone.2010.03.023. View

Yan C, Hao L, Hussein A, Young P . Ti-6Al-4V triply periodic minimal surface structures for bone implants fabricated via selective laser melting. J Mech Behav Biomed Mater. 2015; 51:61-73. DOI: 10.1016/j.jmbbm.2015.06.024. View

Hong Y, Huber A, Takanari K, Amoroso N, Hashizume R, Badylak S . Mechanical properties and in vivo behavior of a biodegradable synthetic polymer microfiber-extracellular matrix hydrogel biohybrid scaffold. Biomaterials. 2011; 32(13):3387-94. PMC: 3184831. DOI: 10.1016/j.biomaterials.2011.01.025. View

Gugala Z, Gogolewski S . Healing of critical-size segmental bone defects in the sheep tibiae using bioresorbable polylactide membranes. Injury. 2002; 33 Suppl 2:B71-6. DOI: 10.1016/s0020-1383(02)00135-3. View

Zhao L, Weir M, Xu H . An injectable calcium phosphate-alginate hydrogel-umbilical cord mesenchymal stem cell paste for bone tissue engineering. Biomaterials. 2010; 31(25):6502-10. PMC: 3001248. DOI: 10.1016/j.biomaterials.2010.05.017. View

Muller P, Bulnheim U, Diener A, Luthen F, Teller M, Klinkenberg E . Calcium phosphate surfaces promote osteogenic differentiation of mesenchymal stem cells. J Cell Mol Med. 2008; 12(1):281-91. PMC: 3823489. DOI: 10.1111/j.1582-4934.2007.00103.x. View

10.

Yassin M, Leknes K, Pedersen T, Xing Z, Sun Y, Lie S . Cell seeding density is a critical determinant for copolymer scaffolds-induced bone regeneration. J Biomed Mater Res A. 2015; 103(11):3649-58. PMC: 4744655. DOI: 10.1002/jbm.a.35505. View

11.

Lichte P, Pape H, Pufe T, Kobbe P, Fischer H . Scaffolds for bone healing: concepts, materials and evidence. Injury. 2011; 42(6):569-73. DOI: 10.1016/j.injury.2011.03.033. View

12.

Kumar A, Nune K, Basu B, Misra R . Mechanistic contribution of electroconductive hydroxyapatite-titanium disilicide composite on the alignment and proliferation of cells. J Biomater Appl. 2016; 30(10):1505-16. DOI: 10.1177/0885328216631670. View

13.

Dupont K, Sharma K, Stevens H, Boerckel J, Garcia A, Guldberg R . Human stem cell delivery for treatment of large segmental bone defects. Proc Natl Acad Sci U S A. 2010; 107(8):3305-10. PMC: 2840521. DOI: 10.1073/pnas.0905444107. View

14.

Yanez A, Herrera A, Martel O, Monopoli D, Afonso H . Compressive behaviour of gyroid lattice structures for human cancellous bone implant applications. Mater Sci Eng C Mater Biol Appl. 2016; 68:445-448. DOI: 10.1016/j.msec.2016.06.016. View

15.

Chen Y, Xu J, Huang Z, Yu M, Zhang Y, Chen H . An Innovative Approach for Enhancing Bone Defect Healing Using PLGA Scaffolds Seeded with Extracorporeal-shock-wave-treated Bone Marrow Mesenchymal Stem Cells (BMSCs). Sci Rep. 2017; 7:44130. PMC: 5341040. DOI: 10.1038/srep44130. View

16.

Luo F, Hou T, Zhang Z, Xie Z, Wu X, Xu J . Effects of initial cell density and hydrodynamic culture on osteogenic activity of tissue-engineered bone grafts. PLoS One. 2013; 8(1):e53697. PMC: 3543387. DOI: 10.1371/journal.pone.0053697. View

17.

Wang M, Vorwald C, Dreher M, Mott E, Cheng M, Cinar A . Evaluating 3D-printed biomaterials as scaffolds for vascularized bone tissue engineering. Adv Mater. 2014; 27(1):138-44. PMC: 4404492. DOI: 10.1002/adma.201403943. View

18.

Wong R, Ashton M, Dodou K . Effect of Crosslinking Agent Concentration on the Properties of Unmedicated Hydrogels. Pharmaceutics. 2015; 7(3):305-19. PMC: 4588202. DOI: 10.3390/pharmaceutics7030305. View

19.

Eglin D, Maalheem S, Livage J, Coradin T . In vitro apatite forming ability of type I collagen hydrogels containing bioactive glass and silica sol-gel particles. J Mater Sci Mater Med. 2006; 17(2):161-7. DOI: 10.1007/s10856-006-6820-6. View

20.

Zhao F, Grayson W, Ma T, Bunnell B, Lu W . Effects of hydroxyapatite in 3-D chitosan-gelatin polymer network on human mesenchymal stem cell construct development. Biomaterials. 2005; 27(9):1859-67. DOI: 10.1016/j.biomaterials.2005.09.031. View