Molecular Typing of of Different Origins Based on the Polymorphism of the Gene: Characterization of a Novel Type

The present study was conducted to determine the molecular diversity of strains isolated from human, bovine and food samples based on the polymorphism of the gene. A total of 208 isolated from human, bovine raw milk and food samples were assessed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and single locus sequence typing (SLST) methods, followed by determination of types using Ridom SpaServer. Altogether, 15 distinct RFLP patterns were recorded (I-XV). The highest heterogeneity was observed among isolated from humans, whereas most of bovine and food isolates indicated certain RFLP patterns. Although most of the isolates from patients showed RFLP pattern I, none of the isolated from carriers had this pattern. Besides, the results of SLST led to the characterization of 16 types, and one of them was a novel type which has been registered in Ridom SpaServer for the first time and designated as type t16929. Determination of a high number of shared RFLP patterns between human and food isolates indicated possible transmission of and the source of food contamination. Thus, effective hygiene measures should be taken to break transmission routes. However, it seems that isolated from patients, carriers and bovine should be considered in a different way, since some isolates had similar patterns, while the others showed their own specific pattern.