A Flexible System for Cultivation of and Other Formate-Utilizing Methanogens

Overview

Journal Archaea

Specialty Microbiology

Date 2018 Jan 20

PMID 29348732

Citations 17

Authors

Feng Long

Liangliang Wang

Boguslaw Lupa

William B Whitman

Affiliations

Soon will be listed here.

Abstract

Many hydrogenotrophic methanogens use either H or formate as the major electron donor to reduce CO for methane production. The conventional cultivation of these organisms uses H and CO as the substrate with frequent replenishment of gas during growth. H is explosive and requires an expensive gassing system to handle safely. Formate is as an ideal alternative substrate from the standpoints of both economy and safety but leads to large changes in the culture pH during growth. Here, we report that glycylglycine is an inexpensive and nontoxic buffer suitable for growth of and . This cultivation system is suitable for growth on liquid as well as solid medium in serum bottles. Moreover, it allows cultivation of liter scale cultures without expensive fermentation equipment. This formate cultivation system provides an inexpensive and flexible alternative for the growth of formate-utilizing, hydrogenotrophic methanogens.

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