Viral Highway to Nucleus Exposed by Image Correlation Analyses

Overview

Journal Sci Rep

Specialty Science

Date 2018 Jan 20

PMID 29348472

Citations 9

Authors

Elina Mantyla

Jenu V Chacko

Vesa Aho

Colin R Parrish

Victor Shahin

Michael Kann

Michelle A Digman

Enrico Gratton

Maija Vihinen-Ranta

Affiliations

Soon will be listed here.

Abstract

Parvoviral genome translocation from the plasma membrane into the nucleus is a coordinated multistep process mediated by capsid proteins. We used fast confocal microscopy line scan imaging combined with image correlation methods including auto-, pair- and cross-correlation, and number and brightness analysis, to study the parvovirus entry pathway at the single-particle level in living cells. Our results show that the endosome-associated movement of virus particles fluctuates from fast to slow. Fast transit of single cytoplasmic capsids to the nuclear envelope is followed by slow movement of capsids and fast diffusion of capsid fragments in the nucleoplasm. The unique combination of image analyses allowed us to follow the fate of intracellular single virus particles and their interactions with importin β revealing previously unknown dynamics of the entry pathway.

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