Extract from an Established Cell Culture Stimulates Skin Wound Healing

Overview

Journal Biomed Res Int

Publisher Wiley

Specialties Biomedical Engineering
Biotechnology
General Medicine

Date 2018 Jan 16

PMID 29333453

Citations 11

Authors

O di Martino

A Tito

A De Lucia

A Cimmino

F Cicotti

F Apone

G Colucci

V Calabro

Affiliations

Soon will be listed here.

Abstract

Higher plants are the source of a wide array of bioactive compounds that support skin integrity and health. , family Malvaceae, is a plant of Chinese origin known for its antipyretic, anthelmintic, and antifungal properties. The aim of this study was to assess the healing and hydration properties of ethanolic extract (HSEE). We established a cell culture from leaves and obtained an ethanol soluble extract from cultured cells. The properties of the extract were tested by gene expression and functional analyses on human fibroblast, keratinocytes, and skin explants. HSEE treatment increased the healing potential of fibroblasts and keratinocytes. Specifically, HSEE significantly stimulated fibronectin and collagen synthesis by 16 and 60%, respectively, while fibroblasts contractility was enhanced by 30%. These results were confirmed on skin explants, where HSEE accelerated the wound healing activity in terms of epithelium formation and fibronectin production. Moreover, HSEE increased the expression of genes involved in skin hydration and homeostasis. Specifically, aquaporin 3 and filaggrin genes were enhanced by 20 and 58%, respectively. Our data show that HSEE contains compounds capable of stimulating expression of biomarkers relevant to skin regeneration and hydration thereby counteracting molecular pathways leading to skin damage and aging.

Citing Articles

NAD-Driven Sirtuin Activation by Extract: Exploring the Adaptogenic Potential to Promote Skin Longevity.

Di Lorenzo R, Falanga D, Ricci L, Colantuono A, Greco G, Angelillo M Int J Mol Sci. 2024; 25(8).

PMID: 38673866 PMC: 11049886. DOI: 10.3390/ijms25084282.

Plants propagation with its applications in food, pharmaceuticals and cosmetic industries; current scenario and future approaches.

Hasnain A, Naqvi S, Ayesha S, Khalid F, Ellahi M, Iqbal S Front Plant Sci. 2022; 13:1009395.

PMID: 36311115 PMC: 9606719. DOI: 10.3389/fpls.2022.1009395.

Characterization of Polyphenolic Compounds from and Their Effects on Wound-Healing Process.

Martinez-Cuazitl A, Gomez-Garcia M, Hidalgo-Alegria O, Flores O, Nunez-Gastelum J, Martinez E Molecules. 2022; 27(19).

PMID: 36235058 PMC: 9571823. DOI: 10.3390/molecules27196521.

Modeling of the Senescence-Associated Phenotype in Human Skin Fibroblasts.

Gerasymchuk M, Robinson G, Kovalchuk O, Kovalchuk I Int J Mol Sci. 2022; 23(13).

PMID: 35806127 PMC: 9266450. DOI: 10.3390/ijms23137124.

The Modulatory Influence of Plant-Derived Compounds on Human Keratinocyte Function.

Merecz-Sadowska A, Sitarek P, Zajdel K, Kucharska E, Kowalczyk T, Zajdel R Int J Mol Sci. 2021; 22(22).

PMID: 34830374 PMC: 8618348. DOI: 10.3390/ijms222212488.

References

Bhaskar A, Nithya V . Evaluation of the wound-healing activity of Hibiscus rosa sinensis L (Malvaceae) in Wistar albino rats. Indian J Pharmacol. 2012; 44(6):694-8. PMC: 3523494. DOI: 10.4103/0253-7613.103252. View

Amento E, Beck L . TGF-beta and wound healing. Ciba Found Symp. 1991; 157:115-23; discussion 123-9. DOI: 10.1002/9780470514061.ch8. View

Pereda M, Dieamant G, Eberlin S, Werka R, Colombi D, Queiroz M . Expression of differential genes involved in the maintenance of water balance in human skin by Piptadenia colubrina extract. J Cosmet Dermatol. 2010; 9(1):35-43. DOI: 10.1111/j.1473-2165.2009.00458.x. View

Rawlings A, Matts P . Stratum corneum moisturization at the molecular level: an update in relation to the dry skin cycle. J Invest Dermatol. 2005; 124(6):1099-110. DOI: 10.1111/j.1523-1747.2005.23726.x. View

Moscatiello R, Baldan B, Navazio L . Plant cell suspension cultures. Methods Mol Biol. 2012; 953:77-93. DOI: 10.1007/978-1-62703-152-3_5. View

Vivo M, Matarese M, Sepe M, Di Martino R, Festa L, Calabro V . MDM2-mediated degradation of p14ARF: a novel mechanism to control ARF levels in cancer cells. PLoS One. 2015; 10(2):e0117252. PMC: 4344200. DOI: 10.1371/journal.pone.0117252. View

Guo S, DiPietro L . Factors affecting wound healing. J Dent Res. 2010; 89(3):219-29. PMC: 2903966. DOI: 10.1177/0022034509359125. View

Ngo P, Ramalingam P, Phillips J, Furuta G . Collagen gel contraction assay. Methods Mol Biol. 2006; 341:103-9. DOI: 10.1385/1-59745-113-4:103. View

Luccia B, Manzo N, Vivo M, Galano E, Amoresano A, Crescenzi E . A biochemical and cellular approach to explore the antiproliferative and prodifferentiative activity of Aloe arborescens leaf extract. Phytother Res. 2013; 27(12):1819-28. DOI: 10.1002/ptr.4939. View

10.

Tito A, Carola A, Bimonte M, Barbulova A, Arciello S, De Laurentiis F . A tomato stem cell extract, containing antioxidant compounds and metal chelating factors, protects skin cells from heavy metal-induced damages. Int J Cosmet Sci. 2011; 33(6):543-52. DOI: 10.1111/j.1468-2494.2011.00668.x. View

11.

Cao C, Wan S, Jiang Q, Amaral A, Lu S, Hu G . All-trans retinoic acid attenuates ultraviolet radiation-induced down-regulation of aquaporin-3 and water permeability in human keratinocytes. J Cell Physiol. 2007; 215(2):506-16. DOI: 10.1002/jcp.21336. View

12.

Di Costanzo A, Festa L, Roscigno G, Vivo M, Pollice A, Morasso M . A dominant mutation etiologic for human tricho-dento-osseous syndrome impairs the ability of DLX3 to downregulate ΔNp63α. J Cell Physiol. 2011; 226(8):2189-97. DOI: 10.1002/jcp.22553. View

13.

Qin H, Zheng X, Zhong X, Shetty A, Elias P, Bollag W . Aquaporin-3 in keratinocytes and skin: its role and interaction with phospholipase D2. Arch Biochem Biophys. 2011; 508(2):138-43. PMC: 3061340. DOI: 10.1016/j.abb.2011.01.014. View

14.

Midwood K, Williams L, Schwarzbauer J . Tissue repair and the dynamics of the extracellular matrix. Int J Biochem Cell Biol. 2004; 36(6):1031-7. DOI: 10.1016/j.biocel.2003.12.003. View

15.

Cheng Y, Lee S, Harn H, Huang H, Chang W . The extract of Hibiscus syriacus inducing apoptosis by activating p53 and AIF in human lung cancer cells. Am J Chin Med. 2008; 36(1):171-84. DOI: 10.1142/S0192415X08005680. View

16.

Yoon S, Lee K, Kim D, Hwang D, Won K, Lee D . Effect of Absolute From L. Flower on Wound Healing in Keratinocytes. Pharmacogn Mag. 2017; 13(49):85-89. PMC: 5307920. DOI: 10.4103/0973-1296.197644. View

17.

Vivo M, Ranieri M, Sansone F, Santoriello C, Calogero R, Calabro V . Mimicking p14ARF phosphorylation influences its ability to restrain cell proliferation. PLoS One. 2013; 8(1):e53631. PMC: 3538741. DOI: 10.1371/journal.pone.0053631. View

18.

Bubols G, Vianna D, Medina-Remon A, von Poser G, Lamuela-Raventos R, Eifler-Lima V . The antioxidant activity of coumarins and flavonoids. Mini Rev Med Chem. 2012; 13(3):318-34. DOI: 10.2174/138955713804999775. View

19.

Goldner J . A modification of the masson trichrome technique for routine laboratory purposes. Am J Pathol. 2009; 14(2):237-43. PMC: 1964940. View

20.

Kwon S, Hong S, Kim J, Ahn I . Antioxidant properties of heat-treated Hibiscus syriacus. Izv Akad Nauk Ser Biol. 2003; (1):20-1. View