Enhancement of Nucleoside Production in Based on Biosynthetic Pathway Analysis

Overview

Journal Biomed Res Int

Publisher Wiley

Specialties Biomedical Engineering
Biotechnology
General Medicine

Date 2018 Jan 16

PMID 29333435

Citations 4

Authors

Zhi-Qiang Liu

Bo Zhang

Shan Lin

Peter James Baker

Mao-Sheng Chen

Ya-Ping Xue

Hui Wu

Feng Xu

Shui-Jin Yuan

Yi Teng

Ling-Fang Wu

Yu-Guo Zheng

Affiliations

Soon will be listed here.

Abstract

To enhance nucleoside production in , the biosynthetic pathways of purine and pyrimidine nucleosides were constructed and verified. The differential expression analysis showed that , and genes involved in purine nucleotide biosynthesis were significantly upregulated 16.56-fold, 8-fold, and 5.43-fold, respectively. Moreover, , , and genes participating in pyrimidine nucleoside biosynthesis were upregulated 4.53-fold, 10.63-fold, 4.26-fold, and 5.98-fold, respectively. To enhance the nucleoside production, precursors for synthesis of nucleosides were added based on the analysis of biosynthetic pathways. Uridine and cytidine contents, respectively, reached 5.04 mg/g and 3.54 mg/g when adding 2 mg/mL of ribose, resulting in an increase of 28.6% and 296% compared with the control, respectively. Meanwhile, uridine and cytidine contents, respectively, reached 10.83 mg/g 2.12 mg/g when adding 0.3 mg/mL of uracil, leading to an increase of 176.3% and 137.1%, respectively. This report indicated that fermentation regulation was an effective way to enhance the nucleoside production in based on biosynthetic pathway analysis.

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