Uncovering the Evolutionary History of Neo-XY Sex Chromosomes in the Grasshopper Ronderosia Bergii (Orthoptera, Melanoplinae) Through Satellite DNA Analysis

Overview

Journal BMC Evol Biol

Publisher Biomed Central

Specialty Biology

Date 2018 Jan 14

PMID 29329524

Citations 11

Authors

Octavio M Palacios-Gimenez

Diogo Milani

Bernardo Lemos

Elio R Castillo

Dardo A Marti

Erica Ramos

Cesar Martins

Diogo C Cabral-de-Mello

Affiliations

Soon will be listed here.

Abstract

Background: Neo-sex chromosome systems arose independently multiple times in evolution, presenting the remarkable characteristic of repetitive DNAs accumulation. Among grasshoppers, occurrence of neo-XY was repeatedly noticed in Melanoplinae. Here we analyzed the most abundant tandem repeats of R. bergii (2n = 22, neo-XY♂) using deep Illumina sequencing and graph-based clustering in order to address the neo-sex chromosomes evolution.

Results: The analyses revealed ten families of satDNAs comprising about ~1% of the male genome, which occupied mainly C-positive regions of autosomes. Regarding the sex chromosomes, satDNAs were recorded within centromeric or interstitial regions of the neo-X chromosome and four satDNAs occurred in the neo-Y, two of them being exclusive (Rber248 and Rber299). Using a combination of probes we uncovered five well-defined cytological variants for neo-Y, originated by multiple paracentric inversions and satDNA amplification, besides fragmented neo-Y. These neo-Y variants were distinct in frequency between embryos and adult males.

Conclusions: The genomic data together with cytogenetic mapping enabled us to better understand the neo-sex chromosome dynamics in grasshoppers, reinforcing differentiation of neo-X and neo-Y and revealing the occurrence of multiple additional rearrangements involved in the neo-Y evolution of R. bergii. We discussed the possible causes that led to differences in frequency for the neo-Y variants between embryos and adults. Finally we hypothesize about the role of DNA satellites in R. bergii as well as putative historical events involved in the evolution of the R. bergii neo-XY.

Citing Articles

Neo-Sex Chromosome Evolution in Treehoppers Despite Long-Term X Chromosome Conservation.

Palmer Droguett D, Fletcher M, Alston B, Kocher S, Cabral-de-Mello D, Wright A Genome Biol Evol. 2024; 16(12).

PMID: 39657114 PMC: 11662286. DOI: 10.1093/gbe/evae264.

Orthopteran Neo-Sex Chromosomes Reveal Dynamics of Recombination Suppression and Evolution of Supergenes.

Jayaprasad S, Peona V, Ellerstrand S, Rossini R, Bunikis I, Pettersson O Mol Ecol. 2024; 33(23):e17567.

PMID: 39475093 PMC: 11589690. DOI: 10.1111/mec.17567.

On the Origin of Neo-Sex Chromosomes in the Neotropical Dragonflies and (Aeshnidae, Odonata).

Mola L, Vrbova I, Tosto D, Zrzava M, Marec F Insects. 2022; 13(12).

PMID: 36555069 PMC: 9784284. DOI: 10.3390/insects13121159.

Study of four Neotropical species of tree crickets Oecanthus Serville, 1831 (Orthoptera, Gryllidae) using cytogenetic and molecular markers.

Silva A, de Oliveira T, Bertocchi N, Valente V, Zefa E, Depra M Genet Mol Biol. 2022; 45(2):e20210213.

PMID: 35499272 PMC: 9059253. DOI: 10.1590/1678-4685-GMB-2021-0213.

High karyotypic variation in Hagen, 1861 species, with insights about the neo-XY in Calvert, 1909 (Libellulidae, Odonata).

Mola L, Fourastie M, Agopian S Comp Cytogenet. 2021; 15(4):355-374.

PMID: 34804379 PMC: 8580954. DOI: 10.3897/CompCytogen.v15.i4.68761.

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