Spin-echo Based Diagonal Peak Suppression in Solid-state MAS NMR Homonuclear Chemical Shift Correlation Spectra

Overview

Journal J Magn Reson

Publisher Elsevier

Date 2018 Jan 12

PMID 29324246

Authors

Kaiyu Wang

Zhiyong Zhang

Xiaoyan Ding

Fang Tian

Yuqing Huang

Zhong Chen

Riqiang Fu

Affiliations

Soon will be listed here.

Abstract

The feasibility of using the spin-echo based diagonal peak suppression method in solid-state MAS NMR homonuclear chemical shift correlation experiments is demonstrated. A complete phase cycling is designed in such a way that in the indirect dimension only the spin diffused signals are evolved, while all signals not involved in polarization transfer are refocused for cancellation. A data processing procedure is further introduced to reconstruct this acquired spectrum into a conventional two-dimensional homonuclear chemical shift correlation spectrum. A uniformly C, N labeled Fmoc-valine sample and the transmembrane domain of a human protein, LR11 (sorLA), in native Escherichia coli membranes have been used to illustrate the capability of the proposed method in comparison with standard C-C chemical shift correlation experiments.

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