» Articles » PMID: 2932370

Nucleotide Sequence of the GDH Gene Coding for the NADP-specific Glutamate Dehydrogenase of Saccharomyces Cerevisiae

Overview
Journal Gene
Specialty Molecular Biology
Date 1985 Jan 1
PMID 2932370
Citations 25
Authors
Affiliations
Soon will be listed here.
Abstract

The isolation of the Saccharomyces cerevisiae gene for NADP-dependent glutamate dehydrogenase (NADP-GDH) by cross hybridization to the Neurospora crassa am gene, known to encode for NADP-GDH is described. Two DNA fragments selected from a yeast genomic library in phage lambda gt11 were shown by restriction analysis to share 2.5 kb of common sequence. A yeast shuttle vector (CV13) carrying either to the cloned fragments complements the gdh- strain of S. cerevisiae and directs substantial overproduction of NADP-GDH. One of the cloned fragments was sequenced, and the deduced amino acid (aa) sequence of the yeast NADP-GDH is 64% homologous to N. crassa, 51% to Escherichia coli and 24% to bovine NADP-GDHs.

Citing Articles

Endocytosis of nutrient transporters in fungi: The ART of connecting signaling and trafficking.

Barata-Antunes C, Alves R, Talaia G, Casal M, Geros H, Mans R Comput Struct Biotechnol J. 2021; 19:1713-1737.

PMID: 33897977 PMC: 8050425. DOI: 10.1016/j.csbj.2021.03.013.


An Examination by Site-Directed Mutagenesis of Putative Key Residues in the Determination of Coenzyme Specificity in Clostridial NAD-Dependent Glutamate Dehydrogenase.

Griffin J, Engel P Enzyme Res. 2011; 2011:595793.

PMID: 21876794 PMC: 3157743. DOI: 10.4061/2011/595793.


The NADP-dependent glutamate dehydrogenase gene from the astaxanthin producer Xanthophyllomyces dendrorhous: use of Its promoter for controlled gene expression.

Rodriguez-Saiz M, Godio R, Alvarez V, de la Fuente J, Martin J, Barredo J Mol Biotechnol. 2008; 41(2):165-72.

PMID: 19016007 DOI: 10.1007/s12033-008-9123-y.


A simple program to calculate codon bias index.

Wang T, Cheng W, Lee B Mol Biotechnol. 1998; 10(2):103-6.

PMID: 9819810 DOI: 10.1007/BF02760858.


The NADP+-linked glutamate dehydrogenase from Trypanosoma cruzi: sequence, genomic organization and expression.

Barderi P, Campetella O, Frasch A, Santome J, Hellman U, Pettersson U Biochem J. 1998; 330 ( Pt 2):951-8.

PMID: 9480915 PMC: 1219230. DOI: 10.1042/bj3300951.