Fibroblast and Keratinocyte Gene Expression Following Exposure to Extracts of Neem Plant ()

Overview

Journal Data Brief

Date 2018 Jan 12

PMID 29322079

Citations 3

Authors

Takao Someya

Katsura Sano

Kotaro Hara

Yoshimasa Sagane

Toshihiro Watanabe

R G S Wijesekara

Affiliations

Soon will be listed here.

Abstract

This data article provides gene expression profiles, determined by using real-time PCR, of fibroblasts and keratinocytes treated with 0.01% and 0.001% extracts of neem plant (Azadirachta indica), local name "Kohomba" in Sri Lanka, harvested in Sri Lanka. For fibroblasts, the dataset includes expression profiles for genes encoding hyaluronan synthase 1 (HAS1), hyaluronan synthase 2 (HAS2), hyaluronidase-1 (HYAL1), hyaluronidase-2 (HYAL2), versican, aggrecan, CD44, collagen, type I, alpha 1 (COL1A1), collagen, type III, alpha 1 (COL3A1), collagen, type VII, alpha 1 (COL7A1), matrix metalloproteinase 1 (MMP1), acid ceramidase, basic fibroblast growth factor (bFGF), fibroblast growth factor-7 (FGF7), vascular endothelial growth factor (VEGF), interleukin-1 alpha (IL-1α), cyclooxygenase-2 (cox2), transforming growth factor beta (TGF-β), and aquaporin 3 (AQP3). For keratinocytes, the expression profiles are for genes encoding HAS1, HAS2, HYAL1, HYAL2, versican, CD44, IL-1α, cox2, TGF-β, AQP3, Laminin5, collagen, type XVII, alpha 1 (COL17A1), integrin alpha-6 (ITGA6), ceramide synthase 3 (CERS3), elongation of very long chain fatty acids protein 1 (ELOVL1), elongation of very long chain fatty acids protein 4 (ELOVL4), filaggrin (FLG), transglutaminase 1 (TGM1), and keratin 1 (KRT1). The expression profiles are provided as bar graphs.

Citing Articles

Unravelling the Therapeutic Potential of Nano-Delivered Functional Foods in Chronic Respiratory Diseases.

Clarence D, Paudel K, Manandhar B, Singh S, Devkota H, Panneerselvam J Nutrients. 2022; 14(18).

PMID: 36145202 PMC: 9503475. DOI: 10.3390/nu14183828.

Colorectal Cancer Stem Cells in the Progression to Liver Metastasis.

Gonzalez-Villarreal C, Quiroz-Reyes A, Islas J, Garza-Trevino E Front Oncol. 2020; 10:1511.

PMID: 32974184 PMC: 7468493. DOI: 10.3389/fonc.2020.01511.

Data on Spectroscopic, Rheological characterization of neem oil and its isolated fractions.

Bhargava S, Madhav N Data Brief. 2018; 21:996-1003.

PMID: 30426057 PMC: 6222080. DOI: 10.1016/j.dib.2018.10.049.

References

Lolmede K, Durand de Saint Front V, Galitzky J, Lafontan M, Bouloumie A . Effects of hypoxia on the expression of proangiogenic factors in differentiated 3T3-F442A adipocytes. Int J Obes Relat Metab Disord. 2003; 27(10):1187-95. DOI: 10.1038/sj.ijo.0802407. View

Iino M, Ehama R, Nakazawa Y, Iwabuchi T, Ogo M, Tajima M . Adenosine stimulates fibroblast growth factor-7 gene expression via adenosine A2b receptor signaling in dermal papilla cells. J Invest Dermatol. 2007; 127(6):1318-25. DOI: 10.1038/sj.jid.5700728. View

Hou Y, Yuan S, Li H, Wu J, Lu J, Liu G . ERbeta exerts multiple stimulative effects on human breast carcinoma cells. Oncogene. 2004; 23(34):5799-806. DOI: 10.1038/sj.onc.1207765. View

Roth P, Silginer M, Goodman S, Hasenbach K, Thies S, Maurer G . Integrin control of the transforming growth factor-β pathway in glioblastoma. Brain. 2013; 136(Pt 2):564-76. DOI: 10.1093/brain/aws351. View

Bove P, Grubb B, Okada S, Ribeiro C, Rogers T, Randell S . Human alveolar type II cells secrete and absorb liquid in response to local nucleotide signaling. J Biol Chem. 2010; 285(45):34939-49. PMC: 2966108. DOI: 10.1074/jbc.M110.162933. View

Ling H, Sylvestre J, Jolicoeur P . Notch1-induced mammary tumor development is cyclin D1-dependent and correlates with expansion of pre-malignant multipotent duct-limited progenitors. Oncogene. 2010; 29(32):4543-54. DOI: 10.1038/onc.2010.186. View

Perrotti V, Palmieri A, Pellati A, Degidi M, Ricci L, Piattelli A . Effect of titanium surface topographies on human bone marrow stem cells differentiation in vitro. Odontology. 2012; 101(2):133-9. DOI: 10.1007/s10266-012-0067-0. View

Maeda-Sano K, Gotoh M, Morohoshi T, Someya T, Murofushi H, Murakami-Murofushi K . Cyclic phosphatidic acid and lysophosphatidic acid induce hyaluronic acid synthesis via CREB transcription factor regulation in human skin fibroblasts. Biochim Biophys Acta. 2014; 1841(9):1256-63. DOI: 10.1016/j.bbalip.2014.05.004. View

Zhang Z, Zhang J, Miao L, Liu K, Yang S, Pan C . Interleukin-11 promotes the progress of gastric carcinoma via abnormally expressed versican. Int J Biol Sci. 2012; 8(3):383-93. PMC: 3291855. DOI: 10.7150/ijbs.3579. View

10.

Gomez-Leduc T, Hervieu M, Legendre F, Bouyoucef M, Gruchy N, Poulain L . Chondrogenic commitment of human umbilical cord blood-derived mesenchymal stem cells in collagen matrices for cartilage engineering. Sci Rep. 2016; 6:32786. PMC: 5015060. DOI: 10.1038/srep32786. View

11.

Rock K, Grandoch M, Majora M, Krutmann J, Fischer J . Collagen fragments inhibit hyaluronan synthesis in skin fibroblasts in response to ultraviolet B (UVB): new insights into mechanisms of matrix remodeling. J Biol Chem. 2011; 286(20):18268-76. PMC: 3093899. DOI: 10.1074/jbc.M110.201665. View

12.

Kypriotou M, Beauchef G, Chadjichristos C, Widom R, Renard E, Jimenez S . Human collagen Krox up-regulates type I collagen expression in normal and scleroderma fibroblasts through interaction with Sp1 and Sp3 transcription factors. J Biol Chem. 2007; 282(44):32000-14. DOI: 10.1074/jbc.M705197200. View

13.

Knaup J, Gruber C, Krammer B, Ziegler V, Bauer J, Verwanger T . TGFβ-signaling in squamous cell carcinoma occurring in recessive dystrophic epidermolysis bullosa. Anal Cell Pathol (Amst). 2011; 34(6):339-53. PMC: 4605790. DOI: 10.3233/ACP-2011-0039. View

14.

Shibata A, Nakagawa K, Kawakami Y, Tsuzuki T, Miyazawa T . Suppression of gamma-tocotrienol on UVB induced inflammation in HaCaT keratinocytes and HR-1 hairless mice via inflammatory mediators multiple signaling. J Agric Food Chem. 2010; 58(11):7013-20. DOI: 10.1021/jf100691g. View

15.

Daniela L, Alla P, Maurelli R, Elena D, Giovanna P, Vladimir K . Anti-inflammatory effects of concentrated ethanol extracts of Edelweiss (Leontopodium alpinum Cass.) callus cultures towards human keratinocytes and endothelial cells. Mediators Inflamm. 2012; 2012:498373. PMC: 3474292. DOI: 10.1155/2012/498373. View

16.

Anderson R, Sciorio R, Kinnell H, Bayne R, Thong K, Sousa P . Cumulus gene expression as a predictor of human oocyte fertilisation, embryo development and competence to establish a pregnancy. Reproduction. 2009; 138(4):629-37. DOI: 10.1530/REP-09-0144. View

17.

Rock K, Meusch M, Fuchs N, Tigges J, Zipper P, Fritsche E . Estradiol protects dermal hyaluronan/versican matrix during photoaging by release of epidermal growth factor from keratinocytes. J Biol Chem. 2012; 287(24):20056-69. PMC: 3370189. DOI: 10.1074/jbc.M112.353151. View

18.

Twarock S, Tammi M, Savani R, Fischer J . Hyaluronan stabilizes focal adhesions, filopodia, and the proliferative phenotype in esophageal squamous carcinoma cells. J Biol Chem. 2010; 285(30):23276-84. PMC: 2906320. DOI: 10.1074/jbc.M109.093146. View