Assessment of Real-Time Polymerase Chain Reaction for the Detection of Spp. DNA from Human Fecal Samples

Overview

Journal Am J Trop Med Hyg

Specialty Tropical Medicine

Date 2018 Jan 10

PMID 29313482

Citations 3

Authors

Francesca Perandin

Elena Pomari

Camilla Bonizzi

Manuela Mistretta

Fabio Formenti

Zeno Bisoffi

Affiliations

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Abstract

Sporadic cases of Tricostrongylosis are reported in humans. Diagnosis of enteric relies primarily on coproscopic analysis but morphological identification is difficult because of similarity among nematode species. The method is time consuming and requires some expertise. To overcome these limitations, we developed a molecular approach by real-time polymerase chain reaction (PCR) to provide a rapid, specific, and sensitive tool to detect spp. in human feces. We designed primers and probe specific for rDNA region 5.8S and internal transcribed spacer 2. Three Italian family clusters were analyzed and DNA sequencing was performed to confirm real-time PCR results comparing with known GenBank sequence data. Sequence analysis showed ≥ 99% identity to and . This study provides a molecular methodology suitable for fast and specific detection of in fecal specimens and to distinguish the zoonotic species.

Citing Articles

Comparison of Molecular and Parasitological Methods for Diagnosis of Human Trichostrongylosis.

Pandi M, Sharifdini M, Ashrafi K, Atrkar Roushan Z, Rahmati B, Hajipour N Front Cell Infect Microbiol. 2021; 11:759396.

PMID: 34722344 PMC: 8548760. DOI: 10.3389/fcimb.2021.759396.

Concomitant Infection of and Intestinal Parasites in Adults Attending a Referral Centre for Parasitic Infections in North Eastern Italy.

Pomari E, Ursini T, Silva R, Leonardi M, Ligozzi M, Angheben A J Clin Med. 2020; 9(8).

PMID: 32722134 PMC: 7465117. DOI: 10.3390/jcm9082366.

Assessment of real-time PCR for Helicobacter pylori DNA detection in stool with co-infection of intestinal parasites: a comparative study of DNA extraction methods.

Leonardi M, La Marca G, Pajola B, Perandin F, Ligozzi M, Pomari E BMC Microbiol. 2020; 20(1):131.

PMID: 32448186 PMC: 7247253. DOI: 10.1186/s12866-020-01824-5.

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