» Articles » PMID: 29312492

MicroRNA-346 Regulates Neural Stem Cell Proliferation and Differentiation by Targeting KLF4

Overview
Journal Am J Transl Res
Specialty General Medicine
Date 2018 Jan 10
PMID 29312492
Citations 15
Authors
Affiliations
Soon will be listed here.
Abstract

MicroRNAs have been shown to play an important role in stem cell fate determination and self-renewal. However, the role of miRNAs in neural stem cells (NSCs) remains poorly understood. In this study, we showed that miR-346, a less characterized microRNA, promoted NSCs proliferation, differentiation and apoptosis by targeting KLF4, a core transcriptional factor in stem cell fate determination. Our data suggested that miR-346 could directly target the 3'-untranslated region of KLF4. Overexpression of miR-346 decreased KLF4 expression at both mRNA and protein levels in NSCs. More importantly, Overexpression of miR-346 repressed NSC proliferation and induced the expression of lineage markers including GFAP and Tuj1. Additionally, overexpression of miR-346 promoted apoptosis of NSCs. In concert, suppressing its expression by an antisense RNA, anti-miR-346, promoted NSC proliferation, and meanwhile inhibited its differentiation and apoptosis. We also showed that the effects of miR-346 overexpression could be reversed by re-expression of KLF4. Taken together, Those data suggest that miR-346 is a novel miRNA that regulates NSC proliferation and differentiation by targeting KLF4.

Citing Articles

Natural compounds as regulators of miRNAs: exploring a new avenue for treating brain cancer.

Doghish A, El-Dakroury W, Abulsoud A, Abdelmaksoud N, Aly S, Elbadry A Naunyn Schmiedebergs Arch Pharmacol. 2025; .

PMID: 40047858 DOI: 10.1007/s00210-025-03934-1.


Human iPSC-derived neural stem cells displaying radial glia signature exhibit long-term safety in mice.

Luciani M, Garsia C, Beretta S, Cifola I, Peano C, Merelli I Nat Commun. 2024; 15(1):9433.

PMID: 39487141 PMC: 11530573. DOI: 10.1038/s41467-024-53613-7.


Krüppel-like factors family in health and disease.

Xiang T, Yang C, Deng Z, Sun D, Luo F, Chen Y MedComm (2020). 2024; 5(9):e723.

PMID: 39263604 PMC: 11387732. DOI: 10.1002/mco2.723.


The role of microRNA-325-3p as a critical player in cell death in NSCs and astrocytes.

Lee Y, Lee S, Jeong D, Lee H, Ko K Front Cell Dev Biol. 2024; 11:1223987.

PMID: 38379959 PMC: 10877600. DOI: 10.3389/fcell.2023.1223987.


Therapeutic Targeting of Krüppel-Like Factor 4 and Its Pharmacological Potential in Parkinson's Disease: a Comprehensive Review.

Zamanian M, Golmohammadi M, Amin R, Bustani G, Romero-Parra R, Zabibah R Mol Neurobiol. 2023; 61(6):3596-3606.

PMID: 37996730 PMC: 11087351. DOI: 10.1007/s12035-023-03800-2.


References
1.
Wang J, Wang B, Chen L, Yang J, Gong Z, Zhao X . miR-10b promotes invasion by targeting KLF4 in osteosarcoma cells. Biomed Pharmacother. 2016; 84:947-953. DOI: 10.1016/j.biopha.2016.09.108. View

2.
Zhao L, Han T, Li Y, Sun J, Zhang S, Liu Y . The lncRNA SNHG5/miR-32 axis regulates gastric cancer cell proliferation and migration by targeting KLF4. FASEB J. 2016; 31(3):893-903. DOI: 10.1096/fj.201600994R. View

3.
He L, Hannon G . MicroRNAs: small RNAs with a big role in gene regulation. Nat Rev Genet. 2004; 5(7):522-31. DOI: 10.1038/nrg1379. View

4.
Nakagawa M, Koyanagi M, Tanabe K, Takahashi K, Ichisaka T, Aoi T . Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts. Nat Biotechnol. 2007; 26(1):101-6. DOI: 10.1038/nbt1374. View

5.
Maherali N, Sridharan R, Xie W, Utikal J, Eminli S, Arnold K . Directly reprogrammed fibroblasts show global epigenetic remodeling and widespread tissue contribution. Cell Stem Cell. 2008; 1(1):55-70. DOI: 10.1016/j.stem.2007.05.014. View