The Recovery of Added Nematode Eggs from Horse and Sheep Faeces by Three Methods

Overview

Journal BMC Vet Res

Publisher Biomed Central

Specialty Veterinary Medicine

Date 2018 Jan 7

PMID 29304858

Citations 13

Authors

Antonio Bosco

Maria Paola Maurelli

Davide Ianniello

Maria Elena Morgoglione

Alessandra Amadesi

Gerald C Coles

Giuseppe Cringoli

Laura Rinaldi

Affiliations

Soon will be listed here.

Abstract

Background: Nematode infections in horses are widespread across the world. Increasing levels of anthelmintic resistance, reported worldwide in equine parasites, have led to the creation of programs for the control of nematodes based on faecal egg counts (FEC). To improve nematode egg counting in equine faecal samples and establish whether the matrix of equine faeces or the eggs affect the counts, the analytical sensitivity, accuracy and precision of Mini-FLOTAC (combined with Fill-FLOTAC), McMaster and Cornell-Wisconsin techniques were compared. Known numbers of eggs extracted from equine or ovine faeces were added to egg free ovine and equine faeces to give counts of 10, 50, 200 and 500 eggs per gram (EPG) of faeces.

Results: The Cornell-Wisconsin significantly underestimated egg counts and McMaster showed a low analytical sensitivity, revealing 100% of sensitivity only for concentrations greater than 200 EPG. EPG values detected by Mini-FLOTAC did not differ significantly from expected counts at any level of egg density.

Conclusions: Mini-FLOTAC combined to Fill-FLOTAC which provides an accurate method of weighing without need for a balance and filtering out debris, could be used for FEC on the farm as well as in the laboratory.

Citing Articles

Comparative assessment of Mini-FLOTAC, McMaster and semi-quantitative flotation for helminth egg examination in camel faeces.

Mohammedsalih K, Hassan S, Juma F, Saeed S, Bashar A, von Samson-Himmelstjerna G Parasit Vectors. 2025; 18(1):5.

PMID: 39800725 PMC: 11726973. DOI: 10.1186/s13071-024-06637-3.

Automating parasite egg detection: insights from the first AI-KFM challenge.

Capuozzo S, Marrone S, Gravina M, Cringoli G, Rinaldi L, Maurelli M Front Artif Intell. 2024; 7:1325219.

PMID: 39268195 PMC: 11390596. DOI: 10.3389/frai.2024.1325219.

Evaluation of Fecal Egg Count Tests for Effective Control of Equine Intestinal Strongyles.

Lejeune M, Mann S, White H, Maguire D, Hazard J, Young R Pathogens. 2023; 12(11).

PMID: 38003748 PMC: 10674696. DOI: 10.3390/pathogens12111283.

and anthelmintic efficacy of peppermint ( L.) essential oil against gastrointestinal nematodes of sheep.

Strbac F, Krnjajic S, Stojanovic D, Ratajac R, Simin N, Orcic D Front Vet Sci. 2023; 10:1232570.

PMID: 37662995 PMC: 10472939. DOI: 10.3389/fvets.2023.1232570.

Comparison of Mini-FLOTAC, Flukefinder and sedimentation techniques for detection and quantification of Fasciola hepatica and Calicophoron daubneyi eggs using spiked and naturally infected bovine faecal samples.

Bosco A, Ciuca L, Maurelli M, Vitiello P, Cringoli G, Prada J Parasit Vectors. 2023; 16(1):260.

PMID: 37533114 PMC: 10399002. DOI: 10.1186/s13071-023-05890-2.

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