Stromal Cell Extracellular Vesicular Cargo Mediated Regulation of Breast Cancer Cell Metastasis Via Ubiquitin Conjugating Enzyme E2 N Pathway

Overview

Journal Oncotarget

Specialty Oncology

Date 2018 Jan 5

PMID 29299114

Citations 23

Authors

Krishna C Vallabhaneni

Patrice Penfornis

Fei Xing

Yoni Hassler

Kristen V Adams

Yin-Yuan Mo

Kounosuke Watabe

Radhika Pochampally

Affiliations

Soon will be listed here.

Abstract

Mesenchymal stromal cells (hMSCs) have been used to understand the stromal cell properties in solid tumors because of their ablity to differentiate into most cell types. We investigated the role of EVs from hMSCs (hMSC-EVs) in breast cancer metastasis using MDA-MB-231 parental cell line and organotropic sub-lines. We demonstrated that hMSC-EVs significantly suppressed the metastatic potential of the parental cell line when compared to their organotropic sublines. hMSC-EVs induce dormancy in the parental cell line but not in their organotropic sub-lines and miR-205 and miR-31 from EV cargo played a role. Further, Ubiquitin Conjugating Enzyme E2 N (UBE2N/Ubc13) - metastasis-regulating gene, is a target of these miRNAs and silencing of UBE2N/Ubc13 expression significantly suppressed migration, invasion, and proliferation of breast cancer cells. To summarize, hMSC-EVs support primary breast tumor progression but suppress the metastasis of breast cancer cells that are not organ-committed through the UBE2N/Ubc13 pathway and play a role in premetastic niche formation.

Citing Articles

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