Modulation of Radiation-induced Base Excision Repair Pathway Gene Expression by Melatonin

Overview

Journal J Med Phys

Date 2018 Jan 4

PMID 29296039

Citations 13

Authors

Saeed Rezapoor

Alireza Shirazi

Sakineh Abbasi

Javad Tavakkoly Bazzaz

Pantea Izadi

Hamed Rezaeejam

Majid Valizadeh

Farid Soleimani-Mohammadi

Masoud Najafi

Affiliations

Soon will be listed here.

Abstract

Objective: Approximately 70% of all cancer patients receive radiotherapy. Although radiotherapy is effective in killing cancer cells, it has adverse effects on normal cells as well. Melatonin (MLT) as a potent antioxidant and anti-inflammatory agent has been proposed to stimulate DNA repair capacity. We investigated the capability of MLT in the modification of radiation-induced DNA damage in rat peripheral blood cells.

Materials And Methods: In this experimental study, male rats ( = 162) were divided into 27 groups (n = 6 in each group) including: irradiation only, vehicle only, vehicle with irradiation, 100 mg/kg MLT alone, 100 mg/kg MLT plus irradiation in 3 different time points, and control. Subsequently, they were irradiated with a single whole-body X-ray radiation dose of 2 and 8 Gy at a dose rate of 200 MU/min. Rats were given an intraperitoneal injection of MLT or the same volume of vehicle alone 1 h prior to irradiation. Blood samples were also taken 8, 24, and 48 h postirradiation, in order to measure the 8-oxoguanine glycosylase1 (), , and expression using quantitative real-time-polymerase chain reaction.

Results: Exposing to the ionizing radiation resulted in downregulation of , , and gene expression. The most obvious suppression was observed in 8 h after exposure. Pretreatments with MLT were able to upregulate these genes when compared to the irradiation-only and vehicle plus irradiation groups ( < 0.05) in all time points.

Conclusion: Our results suggested that MLT in mentioned dose may result in modulation of , , and gene expression in peripheral blood cells to reduce X-ray irradiation-induced DNA damage. Therefore, administration of MLT may increase the normal tissue tolerance to radiation through enhancing the cell DNA repair capacity. We believed that MLT could play a radiation toxicity reduction role in patients who have undergone radiation treatment as a part of cancer radiotherapy.

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