Overexpression of Map3k7 Activates Sinoatrial Node-like Differentiation in Mouse ES-derived Cardiomyocytes

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2017 Dec 28

PMID 29281682

Citations 8

Authors

Kemar Brown

Stephanie Legros

Francis A Ortega

Yunkai Dai

Michael Xavier Doss

David J Christini

Richard B Robinson

Ann C Foley

Affiliations

Soon will be listed here.

Abstract

In vivo, cardiomyocytes comprise a heterogeneous population of contractile cells defined by unique electrophysiologies, molecular markers and morphologies. The mechanisms directing myocardial cells to specific sub-lineages remain poorly understood. Here we report that overexpression of TGFβ-Activated Kinase (TAK1/Map3k7) in mouse embryonic stem (ES) cells faithfully directs myocardial differentiation of embryoid body (EB)-derived cardiac cells toward the sinoatrial node (SAN) lineage. Most cardiac cells in Map3k7-overexpressing EBs adopt markers, cellular morphologies, and electrophysiological behaviors characteristic of the SAN. These data, in addition to the fact that Map3k7 is upregulated in the sinus venous-the source of cells for the SAN-suggest that Map3k7 may be an endogenous regulator of the SAN fate.

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