Molecular Mapping of , a Novel Locus Conferring Resistance to Powdery Mildew in Pepper ()

Overview

Journal Front Plant Sci

Date 2017 Dec 26

PMID 29276524

Citations 8

Authors

Jinkwan Jo

Jelli Venkatesh

Koeun Han

Hea-Young Lee

Gyung Ja Choi

Hee Jae Lee

Doil Choi

Byoung-Cheorl Kang

Affiliations

Soon will be listed here.

Abstract

Powdery mildew, caused by , is a major fungal disease affecting greenhouse-grown pepper (). Powdery mildew resistance has a complex mode of inheritance. In the present study, we investigated a novel powdery mildew resistance locus, , using two mapping populations: 102 'VK515' F families (derived from a cross between resistant parental line 'VK515R' and susceptible parental line 'VK515S') and 80 'PM Singang' F plants (derived from the F 'PM Singang' commercial hybrid). Genetic analysis of the F 'VK515' and F 'PM Singang' populations revealed a single dominant locus for inheritance of the powdery mildew resistance trait. Genetic mapping showed that the locus is located on syntenic regions of pepper chromosome 4 in a 4-Mb region between markers CZ2_11628 and HRM4.1.6 in 'VK515R'. Six molecular markers including one SCAR marker and five SNP markers were localized to a region 0 cM from the locus. Two putative nucleotide-binding site leucine-rich repeat (NBS-LRR)-type disease resistance genes were identified in this region. Genotyping-by-sequencing (GBS) and genetic mapping analysis revealed suppressed recombination in the region, perhaps due to alien introgression. In addition, a comparison of species-specific InDel markers as well as GBS-derived SNP markers indicated that represents a possible source of such alien introgression of powdery mildew resistance into 'VK515R'. The molecular markers developed in this study will be especially helpful for marker-assisted selection in pepper breeding programs for powdery mildew resistance.

Citing Articles

Mapping of resistance genes to powdery mildew based on DNA re-sequencing and bulk segregant analysis in Capsicum.

Zhang T, Bosland P, Ma Y, Wang Y, Li W, Kong W Protoplasma. 2024; .

PMID: 39617838 DOI: 10.1007/s00709-024-02013-1.

Exploring horticultural traits and disease resistance in Capsicum baccatum through segmental introgression lines.

Jo J, Kim G, Back S, Jang S, Kim Y, Han K Theor Appl Genet. 2023; 136(11):233.

PMID: 37878062 DOI: 10.1007/s00122-023-04422-x.

Development and validation of KASP markers for resistance to in L.

Zhang Z, Cao Y, Wang Y, Yu H, Wu H, Liu J Mol Breed. 2023; 43(3):20.

PMID: 37313294 PMC: 10248700. DOI: 10.1007/s11032-023-01367-3.

Chile Pepper () Breeding and Improvement in the "Multi-Omics" Era.

Lozada D, Bosland P, Barchenger D, Haghshenas-Jaryani M, Sanogo S, Walker S Front Plant Sci. 2022; 13:879182.

PMID: 35592583 PMC: 9113053. DOI: 10.3389/fpls.2022.879182.

Diploid chromosome-scale assembly of the Muscadinia rotundifolia genome supports chromosome fusion and disease resistance gene expansion during Vitis and Muscadinia divergence.

Cochetel N, Minio A, Massonnet M, Vondras A, Figueroa-Balderas R, Cantu D G3 (Bethesda). 2021; 11(4).

PMID: 33824960 PMC: 8049426. DOI: 10.1093/g3journal/jkab033.

References

Torii K . Leucine-rich repeat receptor kinases in plants: structure, function, and signal transduction pathways. Int Rev Cytol. 2004; 234:1-46. DOI: 10.1016/S0074-7696(04)34001-5. View

Schiex T, Gaspin C . CARTHAGENE: constructing and joining maximum likelihood genetic maps. Proc Int Conf Intell Syst Mol Biol. 1997; 5:258-67. View

Waterhouse A, Procter J, Martin D, Clamp M, Barton G . Jalview Version 2--a multiple sequence alignment editor and analysis workbench. Bioinformatics. 2009; 25(9):1189-91. PMC: 2672624. DOI: 10.1093/bioinformatics/btp033. View

Liu W, Kang J, Jeong H, Choi H, Yang H, Kim K . Combined use of bulked segregant analysis and microarrays reveals SNP markers pinpointing a major QTL for resistance to Phytophthora capsici in pepper. Theor Appl Genet. 2014; 127(11):2503-13. DOI: 10.1007/s00122-014-2394-8. View

Bai Y, Pavan S, Zheng Z, Zappel N, Reinstadler A, Lotti C . Naturally occurring broad-spectrum powdery mildew resistance in a Central American tomato accession is caused by loss of mlo function. Mol Plant Microbe Interact. 2007; 21(1):30-9. DOI: 10.1094/MPMI-21-1-0030. View

Buschges R, Hollricher K, Panstruga R, Simons G, Wolter M, Frijters A . The barley Mlo gene: a novel control element of plant pathogen resistance. Cell. 1997; 88(5):695-705. DOI: 10.1016/s0092-8674(00)81912-1. View

Pertuze R, Ji Y, Chetelat R . Transmission and recombination of homeologous Solanum sitiens chromosomes in tomato. Theor Appl Genet. 2003; 107(8):1391-401. DOI: 10.1007/s00122-003-1384-z. View

Liu L, Venkatesh J, Jo Y, Koeda S, Hosokawa M, Kang J . Fine mapping and identification of candidate genes for the sy-2 locus in a temperature-sensitive chili pepper (Capsicum chinense). Theor Appl Genet. 2016; 129(8):1541-56. DOI: 10.1007/s00122-016-2723-1. View

Pessina S, Pavan S, Catalano D, Gallotta A, Visser R, Bai Y . Characterization of the MLO gene family in Rosaceae and gene expression analysis in Malus domestica. BMC Genomics. 2014; 15:618. PMC: 4124139. DOI: 10.1186/1471-2164-15-618. View

10.

Xiao S, Ellwood S, Calis O, Patrick E, Li T, Coleman M . Broad-spectrum mildew resistance in Arabidopsis thaliana mediated by RPW8. Science. 2001; 291(5501):118-20. DOI: 10.1126/science.291.5501.118. View

11.

Vallejos C, Jones V, Stall R, Jones J, Minsavage G, Schultz D . Characterization of two recessive genes controlling resistance to all races of bacterial spot in peppers. Theor Appl Genet. 2010; 121(1):37-46. DOI: 10.1007/s00122-010-1289-6. View

12.

Srichumpa P, Brunner S, Keller B, Yahiaoui N . Allelic series of four powdery mildew resistance genes at the Pm3 locus in hexaploid bread wheat. Plant Physiol. 2005; 139(2):885-95. PMC: 1256003. DOI: 10.1104/pp.105.062406. View

13.

Halterman D, Zhou F, Wei F, Wise R, Schulze-Lefert P . The MLA6 coiled-coil, NBS-LRR protein confers AvrMla6-dependent resistance specificity to Blumeria graminis f. sp. hordei in barley and wheat. Plant J. 2001; 25(3):335-48. DOI: 10.1046/j.1365-313x.2001.00982.x. View

14.

Qin C, Yu C, Shen Y, Fang X, Chen L, Min J . Whole-genome sequencing of cultivated and wild peppers provides insights into Capsicum domestication and specialization. Proc Natl Acad Sci U S A. 2014; 111(14):5135-40. PMC: 3986200. DOI: 10.1073/pnas.1400975111. View

15.

Li H, Handsaker B, Wysoker A, Fennell T, Ruan J, Homer N . The Sequence Alignment/Map format and SAMtools. Bioinformatics. 2009; 25(16):2078-9. PMC: 2723002. DOI: 10.1093/bioinformatics/btp352. View

16.

De La Cruz A, Lopez L, Tenllado F, Diaz-Ruiz J, Sanz A, Vaquero C . The coat protein is required for the elicitation of the Capsicum L2 gene-mediated resistance against the tobamoviruses. Mol Plant Microbe Interact. 1997; 10(1):107-13. DOI: 10.1094/MPMI.1997.10.1.107. View

17.

Berzal-Herranz A, De La Cruz A, Tenllado F, Diaz-Ruiz J, Lopez L, Sanz A . The Capsicum L3 gene-mediated resistance against the tobamoviruses is elicited by the coat protein. Virology. 1995; 209(2):498-505. DOI: 10.1006/viro.1995.1282. View

18.

Truong H, Ramos A, Yalcin F, de Ruiter M, van der Poel H, Huvenaars K . Sequence-based genotyping for marker discovery and co-dominant scoring in germplasm and populations. PLoS One. 2012; 7(5):e37565. PMC: 3360789. DOI: 10.1371/journal.pone.0037565. View

19.

Voorrips R, Finkers R, Sanjaya L, Groenwold R . QTL mapping of anthracnose ( Colletotrichum spp.) resistance in a cross between Capsicum annuum and C. chinense. Theor Appl Genet. 2004; 109(6):1275-82. DOI: 10.1007/s00122-004-1738-1. View

20.

Chetelat R, Meglic V, Cisneros P . A genetic map of tomato based on BC(1) Lycopersicon esculentum x Solanum lycopersicoides reveals overall synteny but suppressed recombination between these homeologous genomes. Genetics. 2000; 154(2):857-67. PMC: 1460941. DOI: 10.1093/genetics/154.2.857. View