DEV Induce Autophagy Via the Endoplasmic Reticulum Stress Related Unfolded Protein Response

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2017 Dec 23

PMID 29272280

Citations 18

Authors

Haichang Yin

Lili Zhao

Xinjie Jiang

Siqi Li

Hong Huo

Hongyan Chen

Affiliations

Soon will be listed here.

Abstract

Duck enteritis virus (DEV) can infect ducks, geese, and many other poultry species and leads to acute, septic and highly fatal infectious disease. Autophagy is an evolutionarily ancient pathway that plays an important role in many viral infections. We previously reported that DEV infection induces autophagy for its own benefit, but how this occurs remains unclear. In this study, endoplasmic reticulum (ER) stress was triggered by DEV infection, as demonstrated by the increased expression of the ER stress marker glucose-regulated protein 78 (GRP78) and the dilated morphology of the ER. Pathways associated with the unfolded protein response (UPR), including the PKR-like ER protein kinase (PERK) and inositol-requiring enzyme 1 (IRE1) pathways, but not the activating transcription factor 6 (ATF6) pathway, were activated in DEV-infected duck embryo fibroblast (DEF) cells. In addition, the knockdown of both PERK and IRE1 by small interfering RNAs (siRNAs) reduced the level of LC3-II and viral yields, which suggested that the PERK-eukaryotic initiation factor 2α (eIF2α) and IRE1-x-box protein1 (XBP1) pathways may contribute to DEV-induced autophagy. Collectively, these data offer new insight into the mechanisms of DEV -induced autophagy through activation of the ER stress-related UPR pathway.

Citing Articles

The roles and mechanisms of endoplasmic reticulum stress-mediated autophagy in animal viral infections.

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The cGAS-STING pathway in viral infections: a promising link between inflammation, oxidative stress and autophagy.

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Exosome-Autophagy Crosstalk in Enveloped Virus Infection.

Wang Y, Ren L, Bai H, Jin Q, Zhang L Int J Mol Sci. 2023; 24(13).

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Replication of standard bovine viral diarrhea strain OregonC24Va induces endoplasmic reticulum stress-mediated apoptosis of bovine trophoblast cells.

Yuan N, Song Q, Jin Y, Zhang Z, Wu Z, Sheng X Cell Stress Chaperones. 2022; 28(1):49-60.

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The Multi-Faceted Role of Autophagy During Animal Virus Infection.

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