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Modified Photometric Method for the Determination of Phospholipase A Activities

Overview
Journal Klin Wochenschr
Specialty General Medicine
Date 1989 Feb 1
PMID 2927049
Citations 6
Authors
G E Hoffmann
U Neumann
Affiliations
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Abstract

A modified photometric method for the determination of phospholipase A activities which is based on a previously published reaction principle is described. The modified assay uses a lyophilized substrate emulsion rather than a freshly prepared phospholipid emulsion and a new chromogen (tribromohydroxybenzoic acid and 4-amino-antipyrine) with a high molar absorption coefficient (1,930 m2/mol at 546 nm, 2,900 m2/mol at 512 nm wavelength). The new test is more practicable with respect to pipetting volumes and incubation times. Preliminary results of a method evaluation indicate that the modified assay fulfills the usual criteria for clinical chemical enzyme measurements.

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