Prevalence of Spp. Infections in Hunted Wild Boars in Northern Iran

Overview

Journal Iran J Public Health

Date 2017 Dec 21

PMID 29259947

Citations 6

Authors

Ali Rostami

Hooshang Khazan

Bahram Kazemi

Eshrat Beigom Kia

Mojgan Bandepour

Niloofar Taghipour

Gholamreza Mowlavi

Affiliations

Soon will be listed here.

Abstract

Background: Trichinellosis is an important and neglected foodborne zoonotic infectious disease in worldwide. The most human outbreaks in recent years have been related to consumption of wild boar meat. This cross-sectional study determined the prevalence of spp. infections in hunted wild boars in northern Iran.

Methods: Thirty-five hunted wild boars were subjected in this study in 2015. All samples were examined by conventional artificial digestion method to detect of muscle larvae. Genomic DNA was extracted by phenol-chloroform method from isolated larvae. To identify the species, a PCR-based method was applied using the internal transcribed spacer 2 (ITS2) and mitochondrial small-subunit ribosomal RNA (rRNA) gene sequences.

Results: The overall prevalence of spp. infection was 5.7% (2/35, 95%CI= 0-13.4). The mean larval burdens in two positive samples were 0.05 and 6 larvae per gr tissue muscle, respectively. The PCR reaction, using specific primers, yielded two 367 bp and 195 bp bands on agarose gel for ITS 2 and rrnS, respectively.

Conclusion: There is a hidden burden of spp. infection in wild boar population in Iran. Moreover, is the prevalent species circulating in wild boars of Iran. Therefore, education of the hunters and other consumers should be performed about the risk of consumption of raw or undercooked meat and meat products from wild boars.

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