Conditions and Regulation of Mixed Culture to Promote Shiraia Bambusicola and Phoma Sp. BZJ6 for Laccase Production

Overview

Journal Sci Rep

Specialty Science

Date 2017 Dec 21

PMID 29259312

Citations 8

Authors

Wen Du

Chunlong Sun

Jun Wang

Wenjun Xie

Baoqin Wang

Xuehong Liu

Yumiao Zhang

Yanhui Fan

Affiliations

Soon will be listed here.

Abstract

Mixing cultures induces the biosynthesis of laccase in mixed cells, produces signal molecules, and regulates the production of mixed-cell metabolites. The fungal strain, which promotes laccase production, has been isolated and screened from the host bamboos of endophytic fungi and identified as Phoma sp. BZJ6. When the culture medium is mainly composed of soluble starch, yeast extract, and Phoma sp., the laccase output can reach 4,680 U/L. Nitric oxide (NO) and reactive oxygen species (ROS) were found to promote the regulation of laccase synthesis. Plasma membrane NAD(P)H oxidase inhibitors and NO-specific quenchers can inhibit not only the accumulation of ROS induced and NO synthesis but also the biosynthesis of laccase. The results indicate that the accumulation of superoxide anion radical (O) and hydrogen peroxide (HO) induced by the mixed culture was partially dependent on NO. The mixed culture can also reduce the biomass, increase the synthesis of total phenolics and flavonoids, and enhance the activity of phenylalanine ammonia-lyase and chalcone isomerase. This phenomenon is probably the result of the activated phenylpropanoids-flavonoid pathway. Results confirmed that the mixture culture is advantageous for laccase production and revealed that NO, O, and HO are necessary signal molecules to induce laccase synthesis.

Citing Articles

The Potential Use of Fungal Co-Culture Strategy for Discovery of New Secondary Metabolites.

Xu S, Li M, Hu Z, Shao Y, Ying J, Zhang H Microorganisms. 2023; 11(2).

PMID: 36838429 PMC: 9965835. DOI: 10.3390/microorganisms11020464.

Contrasting regulation of live Bacillus cereus No.1 and its volatiles on Shiraia perylenequinone production.

Xu R, Li X, Zhang X, Shen W, Min C, Wang J Microb Cell Fact. 2022; 21(1):172.

PMID: 35999640 PMC: 9396862. DOI: 10.1186/s12934-022-01897-z.

Coprinopsis cinerea Uses Laccase Lcc9 as a Defense Strategy To Eliminate Oxidative Stress during Fungal-Fungal Interactions.

Liu J, Peng C, Han Q, Wang M, Zhou G, Ye B Appl Environ Microbiol. 2021; 88(1):e0176021.

PMID: 34669425 PMC: 8752157. DOI: 10.1128/AEM.01760-21.

Studies on production and evaluation of biopigment and synthetic dye decolorization capacity of laccase produced by A. oryzae cultivated on agro-waste.

Majumdar S, Bhowal J Bioprocess Biosyst Eng. 2021; 45(1):45-60.

PMID: 34591164 DOI: 10.1007/s00449-021-02638-z.

Enhancement of laccase production by Cerrena unicolor through fungal interspecies interaction and optimum conditions determination.

Kachlishvili E, Jokharidze T, Kobakhidze A, Elisashvili V Arch Microbiol. 2021; 203(7):3905-3917.

PMID: 34014357 DOI: 10.1007/s00203-021-02374-8.

References

Zhang P, Luo Q, Wang R, Xu J . Hydrogen sulfide toxicity inhibits primary root growth through the ROS-NO pathway. Sci Rep. 2017; 7(1):868. PMC: 5429837. DOI: 10.1038/s41598-017-01046-2. View

Du W, Liang J, Han Y, Yu J, Liang Z . Nitric oxide mediates hypocrellin accumulation induced by fungal elicitor in submerged cultures of Shiraia bambusicola. Biotechnol Lett. 2014; 37(1):153-9. DOI: 10.1007/s10529-014-1665-4. View

Yang Y, Shah J, Klessig D . Signal perception and transduction in plant defense responses. Genes Dev. 1997; 11(13):1621-39. DOI: 10.1101/gad.11.13.1621. View

Huff G, Dutta V, Huff W, Rath N . Effects of dietary yeast extract on turkey stress response and heterophil oxidative burst activity. Br Poult Sci. 2011; 52(4):446-55. DOI: 10.1080/00071668.2011.600753. View

Peluso I, Morabito G, Urban L, Ioannone F, Serafini M . Oxidative stress in atherosclerosis development: the central role of LDL and oxidative burst. Endocr Metab Immune Disord Drug Targets. 2012; 12(4):351-60. DOI: 10.2174/187153012803832602. View

Forootanfar H, Rezaei S, Zeinvand-Lorestani H, Tahmasbi H, Mogharabi M, Ameri A . Studies on the laccase-mediated decolorization, kinetic, and microtoxicity of some synthetic azo dyes. J Environ Health Sci Eng. 2016; 14:7. PMC: 4866392. DOI: 10.1186/s40201-016-0248-9. View

Wahl A, Sidorenko Y, Dauner M, Genzel Y, Reichl U . Metabolic flux model for an anchorage-dependent MDCK cell line: characteristic growth phases and minimum substrate consumption flux distribution. Biotechnol Bioeng. 2008; 101(1):135-52. DOI: 10.1002/bit.21873. View

Kannaiyan R, Mahinpey N, Kostenko V, Martinuzzi R . Nutrient media optimization for simultaneous enhancement of the laccase and peroxidases production by coculture of Dichomitus squalens and Ceriporiopsis subvermispora. Biotechnol Appl Biochem. 2014; 62(2):173-85. DOI: 10.1002/bab.1263. View

Ishikawa S, Suzuki K, Fukuda E, Arihara K, Yamamoto Y, Mukai T . Photodynamic antimicrobial activity of avian eggshell pigments. FEBS Lett. 2009; 584(4):770-4. DOI: 10.1016/j.febslet.2009.12.041. View

10.

Feigl G, Lehotai N, Molnar A, Ordog A, Rodriguez-Ruiz M, Palma J . Zinc induces distinct changes in the metabolism of reactive oxygen and nitrogen species (ROS and RNS) in the roots of two Brassica species with different sensitivity to zinc stress. Ann Bot. 2014; 116(4):613-25. PMC: 4577986. DOI: 10.1093/aob/mcu246. View

11.

Zhao Y, Xi Q, Xu Q, He M, Ding J, Dai Y . Correlation of nitric oxide produced by an inducible nitric oxide synthase-like protein with enhanced expression of the phenylpropanoid pathway in Inonotus obliquus cocultured with Phellinus morii. Appl Microbiol Biotechnol. 2015; 99(10):4361-72. DOI: 10.1007/s00253-014-6367-2. View

12.

Du W, Liang Z, Zou X, Han Y, Liang J, Yu J . Effects of microbial elicitor on production of hypocrellin by Shiraia bambusicola. Folia Microbiol (Praha). 2012; 58(4):283-9. DOI: 10.1007/s12223-012-0203-9. View

13.

Baldi A, Jain A, Gupta N, Srivastava A, Bisaria V . Co-culture of arbuscular mycorrhiza-like fungi (Piriformospora indica and Sebacina vermifera) with plant cells of Linum album for enhanced production of podophyllotoxins: a first report. Biotechnol Lett. 2008; 30(9):1671-7. DOI: 10.1007/s10529-008-9736-z. View

14.

Chagas F, Dias L, Pupo M . A mixed culture of endophytic fungi increases production of antifungal polyketides. J Chem Ecol. 2013; 39(10):1335-42. DOI: 10.1007/s10886-013-0351-7. View

15.

Pan K, Zhao N, Yin Q, Zhang T, Xu X, Fang W . Induction of a laccase Lcc9 from Coprinopsis cinerea by fungal coculture and its application on indigo dye decolorization. Bioresour Technol. 2014; 162:45-52. DOI: 10.1016/j.biortech.2014.03.116. View

16.

Meng Y, Liang H, Gao D . [White-rot fungi combinations impact on enzyme productions]. Huan Jing Ke Xue. 2013; 34(1):271-6. View

17.

Foresi N, Mayta M, Lodeyro A, Scuffi D, Correa-Aragunde N, Garcia-Mata C . Expression of the tetrahydrofolate-dependent nitric oxide synthase from the green alga Ostreococcus tauri increases tolerance to abiotic stresses and influences stomatal development in Arabidopsis. Plant J. 2015; 82(5):806-21. DOI: 10.1111/tpj.12852. View

18.

Du W, Sun C, Liang Z, Han Y, Yu J . Antibacterial activity of hypocrellin A against Staphylococcus aureus. World J Microbiol Biotechnol. 2012; 28(11):3151-7. DOI: 10.1007/s11274-012-1125-z. View

19.

Bradford M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72:248-54. DOI: 10.1016/0003-2697(76)90527-3. View

20.

Chang Q, Yan L, Chen M, He H, Qu J . Bactericidal mechanism of Ag/Al2O3 against Escherichia coli. Langmuir. 2007; 23(22):11197-9. DOI: 10.1021/la701006r. View