Detection of PrP and Prion Infectivity in the Ileal Peyer's Patch of Young Calves As Early As 2 months After Oral Challenge with Classical Bovine Spongiform Encephalopathy

Overview

Journal Vet Res

Publisher Biomed Central

Specialty Veterinary Medicine

Date 2017 Dec 21

PMID 29258602

Citations 7

Authors

Ivett Ackermann

Anne Balkema-Buschmann

Reiner Ulrich

Kerstin Tauscher

James C Shawulu

Markus Keller

Olanrewaju I Fatola

Paul Brown

Martin H Groschup

Affiliations

Soon will be listed here.

Abstract

In classical bovine spongiform encephalopathy (C-BSE), an orally acquired prion disease of cattle, the ileal Peyer's patch (IPP) represents the main entry port for the BSE agent. In earlier C-BSE pathogenesis studies, cattle at 4-6 months of age were orally challenged, while there are strong indications that the risk of infection is highest in young animals. In the present study, unweaned calves aged 4-6 weeks were orally challenged to determine the earliest time point at which newly formed PrP and BSE infectivity are detectable in the IPP. For this purpose, calves were culled 1 week as well as 2, 4, 6 and 8 months post-infection (mpi) and IPPs were examined for BSE infectivity using a bovine PrP transgenic mouse bioassay, and for PrP by immunohistochemistry (IHC) and protein misfolding cyclic amplification (PMCA) assays. For the first time, BSE prions were detected in the IPP as early as 2 mpi by transgenic mouse bioassay and PMCA and 4 mpi by IHC in the follicular dendritic cells (FDCs) of the IPP follicles. These data indicate that BSE prions propagate in the IPP of unweaned calves within 2 months of oral uptake of the agent.

Citing Articles

Prion Infectivity and PrP in the Peripheral and Central Nervous System of Cattle 8 Months Post Oral BSE Challenge.

Ackermann I, Ulrich R, Tauscher K, Fatola O, Keller M, Shawulu J Int J Mol Sci. 2021; 22(21).

PMID: 34768738 PMC: 8583047. DOI: 10.3390/ijms222111310.

Exploration of genetic factors resulting in abnormal disease in cattle experimentally challenged with bovine spongiform encephalopathy.

Dudas S, Anderson R, Staskevicus A, Mitchell G, Cross J, Czub S Prion. 2021; 15(1):1-11.

PMID: 33397192 PMC: 7801127. DOI: 10.1080/19336896.2020.1869495.

Evaluation of an alternative method for production of biodiesel from processed fats derived from Category 1, 2 and 3 animal by-products (submitted by College Proteins).

Koutsoumanis K, Allende A, Bolton D, Bover-Cid S, Chemaly M, Davies R EFSA J. 2020; 18(4):e06089.

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Absence of classical and atypical (H- and L-) BSE infectivity in the blood of bovines in the clinical end stage of disease as confirmed by intraspecies blood transfusion.

Balkema-Buschmann A, Ziegler U, Priemer G, Tauscher K, Koster F, Ackermann I J Gen Virol. 2020; 102(1).

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Bovine Spongiform Encephalopathy  - A Review from the Perspective of Food Safety.

Kumagai S, Daikai T, Onodera T Food Saf (Tokyo). 2020; 7(2):21-47.

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