Yi-Qi-Ping-Chuan-Fang Reduces TSLP Elevation Caused by LPS + Poly(I:C) Via Inhibiting TLR4/MYD88/NF-B Signaling Pathway

Overview

Journal Evid Based Complement Alternat Med

Specialty Rehabilitation Medicine

Date 2017 Dec 19

PMID 29250125

Citations 3

Authors

Minye Qu

Xiang Tao

Jian Ma

Affiliations

Soon will be listed here.

Abstract

Objective: To explore the correlation between Thymic Stromal Lymphopoietin (TSLP) and the Nuclear Factor- (NF-) B signaling pathways in bronchial epithelial cells and to clarify whether the traditional Chinese medicine formula Yi-Qi-Ping-Chuan-Fang (YQPC) reduces inflammation by inhibiting TSLP/NF-B signaling pathways.

Methods: Cells were stimulated with LPS + Poly(I:C) and treated with YQPC. The expressions of TSLP and NF-B signaling pathways related proteins P65, IK, IBa, P-P65, P-IK, P-IBa were detected. The effects of NF-B upstream molecules, Toll-like receptors 3 and 4, myeloid differentiation primary response gene 88 (Myd88), TIR-domain-containing adapter-inducing interferon- (TRIF), and downstream inflammatory cytokines, TNF-, IL-1, IL-6, and IL-8, were assessed.

Results: The mRNA and protein expressions of TSLP were significantly increased after LPS + Poly(I:C) stimulation, the total protein IBa and IK decreased ( < 0.05), and the phosphorylated protein P-P65, P-IK, and P-IB increased. After YQPC treatment, the expression of TSLP, P-P65, P-IBa, and P-IK was significantly inhibited ( < 0.05). The activation of TLR4 and MyD88 decreased, and release of IL-1, IL-6, IL-8, and TNF- reduced ( < 0.05).

Conclusion: In summary, the expression of TSLP is activated by the NF-B signaling pathway. YQPC alleviated inflammation by inhibiting TSLP through regulating the NF-B activation and translocation.

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