Ginsenoside Rg3 Decreases Fibrotic and Invasive Nature of Endometriosis by Modulating MiRNA-27b: In Vitro and In Vivo Studies

Overview

Journal Sci Rep

Specialty Science

Date 2017 Dec 17

PMID 29247225

Citations 19

Authors

Min Kyoung Kim

Seung Kyun Lee

Ji Hyun Park

Jae Hoon Lee

Bo Hyon Yun

Joo Hyun Park

Seok Kyo Seo

SiHyun Cho

Young Sik Choi

Affiliations

Soon will be listed here.

Abstract

This research aimed to evaluate the potential therapeutic effects of Rg3 on endometriosis and identify target miRNAs. We designed an in vitro study using human endometrial stromal cells (HESCs) obtained from patients with endometriosis and an in vivo study using mouse models. HESCs were treated with Rg3-enhanced red ginseng extract (Rg3E); real-time PCR and microarray profiling, transfection, and western blot were performed. Mouse endometriosis models were developed and supplemented with Rg3E for 8 weeks. Gross lesion size and fibrotic character were analyzed in the mouse models. RNA levels of Ki-67, col-1, CTGF, fibronectin, TGF-β1, MMP2 and MMP9 significantly decreased in HESCs after Rg3E treatment. Microarray analysis revealed downregulation of miR-27b-3p, which is related to fibrosis modulation. Expression of miR-27b-3p was significantly higher in HESCs from patients with endometriosis than that of controls, and Rg3E treatment significantly decreased its expression; the contraction and migration assay revealed significant reductions in both fibrosis and migration potential in Rg3E-treated HESCs from endometriosis patients. A decrease in size and fibrotic character of endometrial lesions from the Rg3E groups was observed in vivo. In conclusion, Rg3 effectively altered fibrotic properties of HESCs from patients with endometriosis, which is likely associated with miR-27b-3p modulation.

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