Decreased MicroRNA MiR-181c Expression Associated with Gastric Cancer

Overview

Journal J Gastrointest Cancer

Publisher Springer

Specialties Gastroenterology
Oncology

Date 2017 Dec 16

PMID 29243018

Citations 13

Authors

Luanna Munhoz Zabaglia

Nicole Chiuso Bartolomeu

Monica Pezenatto Dos Santos

Rita Luiza Peruquetti

Elizabeth Chen

Marilia de Arruda Cardoso Smith

Spencer Luiz Marques Payao

Lucas Trevizani Rasmussen

Affiliations

Soon will be listed here.

Abstract

Purpose: This study investigated miRNA-181c expression in control patients (healthy gastric mucosa), patients with gastritis, and patients with gastric cancer. The presence of Helicobacter pylori was determined, and the associations between H. pylori infection, levels of miRNA-181c expression, and gastric disease were also analyzed.

Methods: A total of 158 subjects were included in the study, and the three groups were respectively composed of 53 controls, 86 patients with gastritis, and 19 patients with gastric cancer. miRNA-181c expression and H. pylori infection were determined by quantitative real-time PCR and PCR, respectively. The subsequent target gene analysis was performed using the bioinformatics approach to understand the possible mechanisms of gastric cancer.

Results: We determined significantly lower miRNA-181c expression in the gastric cancer group when compared to the control and gastritis groups, regardless of the presence of H. pylori. There was no difference in miRNA-181c expression between the control group and gastritis group, whether the presence of H. pylori was considered or not. The bioinformatics approach identified several genes as possible targets for miRNA-181c, including the X-linked inhibitor of apoptosis (XIAP) gene (which encodes a protein that belongs to a family of apoptotic suppressor proteins), the caspase 9 gene, and the caspase 3 gene. All target genes identified may be involved in gastric cancer and apoptosis pathways.

Conclusion: The results suggest that the presence of H. pylori has no influence on microRNA expression and that the downregulation of miR-181c may play an important role in gastric cancer progression by controlling important genes associated with apoptosis. Therefore, miRNA-181c may be a potential marker of gastric cancer.

Citing Articles

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Epigenetic Regulation of microRNAs in Cancer: Shortening the Distance from Bench to Bedside.

Pajares M, Alemany-Cosme E, Goni S, Bandres E, Palanca-Ballester C, Sandoval J Int J Mol Sci. 2021; 22(14).

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MicroRNAs: The Link between the Metabolic Syndrome and Oncogenesis.

Fodor A, Lazar A, Buchman C, Tiperciuc B, Orasan O, Cozma A Int J Mol Sci. 2021; 22(12).

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miRNAs expression signature potentially associated with lymphatic dissemination in locally advanced prostate cancer.

Pudova E, Krasnov G, Nyushko K, Kobelyatskaya A, Savvateeva M, Poloznikov A BMC Med Genomics. 2020; 13(Suppl 8):129.

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Dysregulated Expression of Apoptosis-Associated Genes and MicroRNAs and Their Involvement in Gastric Carcinogenesis.

Sallas M, Zapparoli D, Santos M, Pereira J, Orcini W, Peruquetti R J Gastrointest Cancer. 2020; 52(2):625-633.

PMID: 32583363 DOI: 10.1007/s12029-019-00353-3.

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