Dual Repression of Endocytic Players by ESCC MicroRNAs and the Polycomb Complex Regulates Mouse Embryonic Stem Cell Pluripotency

Overview

Journal Sci Rep

Specialty Science

Date 2017 Dec 16

PMID 29242593

Citations 5

Authors

Ridim Dadasaheb Mote

Gaurang Mahajan

Anup Padmanabhan

Ramaraju Ambati

Deepa Subramanyam

Affiliations

Soon will be listed here.

Abstract

Cell fate determination in the early mammalian embryo is regulated by multiple mechanisms. Recently, genes involved in vesicular trafficking have been shown to play an important role in cell fate choice, although the regulation of their expression remains poorly understood. Here we demonstrate for the first time that multiple endocytosis associated genes (EAGs) are repressed through a novel, dual mechanism in mouse embryonic stem cells (mESCs). This involves the action of the Polycomb Repressive Complex, PRC2, as well as post-transcriptional regulation by the ESC-specific cell cycle-regulating (ESCC) family of microRNAs. This repression is relieved upon differentiation. Forced expression of EAGs in mESCs results in a decrease in pluripotency, highlighting the importance of dual repression in cell fate regulation. We propose that endocytosis is critical for cell fate choice, and dual repression may function to tightly regulate levels of endocytic genes.

Citing Articles

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Li J, Trivedi V, Diz-Munoz A Semin Cell Dev Biol. 2022; 133:123-134.

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Pluripotency of embryonic stem cells lacking clathrin-mediated endocytosis cannot be rescued by restoring cellular stiffness.

Mote R, Yadav J, Singh S, Tiwari M, V S, Patil S J Biol Chem. 2020; 295(49):16888-16896.

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MicroRNAs reinforce repression of PRC2 transcriptional targets independently and through a feed-forward regulatory network.

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Clathrin-Mediated Endocytosis Regulates a Balance between Opposing Signals to Maintain the Pluripotent State of Embryonic Stem Cells.

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