Community-Acquired Pneumonia in Children: the Challenges of Microbiological Diagnosis

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 2017 Dec 15

PMID 29237789

Citations 35

Authors

C M C Rodrigues

H Groves

Affiliations

Soon will be listed here.

Abstract

Community-acquired pneumonia (CAP) is the leading cause of mortality in children under 5 years of age globally. To improve the management of CAP, we must distinguish CAP from other common pediatric conditions and develop better diagnostic methods to detect the causative organism, so as to best direct appropriate resources in both industrialized and developing countries. Here, we review the diagnostic modalities available for identifying viruses and bacteria in the upper and lower respiratory tract of children, with a discussion of their utility and limitations in diagnosing CAP in children.

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References

Chu H, Englund J, Strelitz B, Lacombe K, Jones C, Follmer K . Rhinovirus Disease in Children Seeking Care in a Tertiary Pediatric Emergency Department. J Pediatric Infect Dis Soc. 2016; 5(1):29-38. PMC: 4765491. DOI: 10.1093/jpids/piu099. View

Malhotra B, Swamy M, Reddy P, Kumar N, Tiwari J . Evaluation of custom multiplex real - time RT - PCR in comparison to fast - track diagnostics respiratory 21 pathogens kit for detection of multiple respiratory viruses. Virol J. 2016; 13:91. PMC: 4896093. DOI: 10.1186/s12985-016-0549-8. View

Iroh Tam P, Bernstein E, Ma X, Ferrieri P . Blood Culture in Evaluation of Pediatric Community-Acquired Pneumonia: A Systematic Review and Meta-analysis. Hosp Pediatr. 2015; 5(6):324-36. DOI: 10.1542/hpeds.2014-0138. View

Gowraiah V, Awasthi S, Kapoor R, Sahana D, Venkatesh P, Gangadhar B . Can we distinguish pneumonia from wheezy diseases in tachypnoeic children under low-resource conditions? A prospective observational study in four Indian hospitals. Arch Dis Child. 2014; 99(10):899-906. DOI: 10.1136/archdischild-2013-305740. View

Brotons P, de Paz H, Esteva C, Latorre I, Munoz-Almagro C . Validation of a loop-mediated isothermal amplification assay for rapid diagnosis of pertussis infection in nasopharyngeal samples. Expert Rev Mol Diagn. 2015; 16(1):125-30. DOI: 10.1586/14737159.2016.1112741. View

Doan Q, Enarson P, Kissoon N, Klassen T, Johnson D . Rapid viral diagnosis for acute febrile respiratory illness in children in the Emergency Department. Cochrane Database Syst Rev. 2012; (5):CD006452. DOI: 10.1002/14651858.CD006452.pub3. View

Liu L, Oza S, Hogan D, Perin J, Rudan I, Lawn J . Global, regional, and national causes of child mortality in 2000-13, with projections to inform post-2015 priorities: an updated systematic analysis. Lancet. 2014; 385(9966):430-40. DOI: 10.1016/S0140-6736(14)61698-6. View

Broccolo F, Falcone V, Esposito S, Toniolo A . Human bocaviruses: Possible etiologic role in respiratory infection. J Clin Virol. 2015; 72:75-81. DOI: 10.1016/j.jcv.2015.09.008. View

Wieser A, Schneider L, Jung J, Schubert S . MALDI-TOF MS in microbiological diagnostics-identification of microorganisms and beyond (mini review). Appl Microbiol Biotechnol. 2011; 93(3):965-74. DOI: 10.1007/s00253-011-3783-4. View

10.

Subramony A, Zachariah P, Krones A, Whittier S, Saiman L . Impact of Multiplex Polymerase Chain Reaction Testing for Respiratory Pathogens on Healthcare Resource Utilization for Pediatric Inpatients. J Pediatr. 2016; 173:196-201.e2. PMC: 5452417. DOI: 10.1016/j.jpeds.2016.02.050. View

11.

Broadbent L, Groves H, Shields M, Power U . Respiratory syncytial virus, an ongoing medical dilemma: an expert commentary on respiratory syncytial virus prophylactic and therapeutic pharmaceuticals currently in clinical trials. Influenza Other Respir Viruses. 2015; 9(4):169-78. PMC: 4474493. DOI: 10.1111/irv.12313. View

12.

Rambaud-Althaus C, Althaus F, Genton B, DAcremont V . Clinical features for diagnosis of pneumonia in children younger than 5 years: a systematic review and meta-analysis. Lancet Infect Dis. 2015; 15(4):439-50. DOI: 10.1016/S1473-3099(15)70017-4. View

13.

Notomi T, Mori Y, Tomita N, Kanda H . Loop-mediated isothermal amplification (LAMP): principle, features, and future prospects. J Microbiol. 2015; 53(1):1-5. DOI: 10.1007/s12275-015-4656-9. View

14.

Wingerter S, Bachur R, Monuteaux M, Neuman M . Application of the world health organization criteria to predict radiographic pneumonia in a US-based pediatric emergency department. Pediatr Infect Dis J. 2012; 31(6):561-4. DOI: 10.1097/INF.0b013e31824da716. View

15.

Jain S, Williams D, Arnold S, Ampofo K, Bramley A, Reed C . Community-acquired pneumonia requiring hospitalization among U.S. children. N Engl J Med. 2015; 372(9):835-45. PMC: 4697461. DOI: 10.1056/NEJMoa1405870. View

16.

Self W, Williams D, Zhu Y, Ampofo K, Pavia A, Chappell J . Respiratory Viral Detection in Children and Adults: Comparing Asymptomatic Controls and Patients With Community-Acquired Pneumonia. J Infect Dis. 2015; 213(4):584-91. PMC: 4721902. DOI: 10.1093/infdis/jiv323. View

17.

Niemz A, Ferguson T, Boyle D . Point-of-care nucleic acid testing for infectious diseases. Trends Biotechnol. 2011; 29(5):240-50. PMC: 3746968. DOI: 10.1016/j.tibtech.2011.01.007. View

18.

Ostergaard M, Nantanda R, Tumwine J, Aabenhus R . Childhood asthma in low income countries: an invisible killer?. Prim Care Respir J. 2012; 21(2):214-9. PMC: 6547929. DOI: 10.4104/pcrj.2012.00038. View

19.

Andrews A, Simpson A, Heine D, Teufel 2nd R . A Cost-Effectiveness Analysis of Obtaining Blood Cultures in Children Hospitalized for Community-Acquired Pneumonia. J Pediatr. 2015; 167(6):1280-6. DOI: 10.1016/j.jpeds.2015.09.025. View

20.

Harris M, Clark J, Coote N, Fletcher P, Harnden A, McKean M . British Thoracic Society guidelines for the management of community acquired pneumonia in children: update 2011. Thorax. 2011; 66 Suppl 2:ii1-23. DOI: 10.1136/thoraxjnl-2011-200598. View